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Gradients in pore size enhance the osteogenic differentiation of human mesenchymal stromal cells in three-dimensional scaffolds

Small fractures in bone tissue can heal by themselves, but in case of larger defects current therapies are not completely successful due to several drawbacks. A possible strategy relies on the combination of additive manufactured polymeric scaffolds and human mesenchymal stromal cells (hMSCs). The a...

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Autores principales: Di Luca, Andrea, Ostrowska, Barbara, Lorenzo-Moldero, Ivan, Lepedda, Antonio, Swieszkowski, Wojcech, Van Blitterswijk, Clemens, Moroni, Lorenzo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4790631/
https://www.ncbi.nlm.nih.gov/pubmed/26961859
http://dx.doi.org/10.1038/srep22898
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author Di Luca, Andrea
Ostrowska, Barbara
Lorenzo-Moldero, Ivan
Lepedda, Antonio
Swieszkowski, Wojcech
Van Blitterswijk, Clemens
Moroni, Lorenzo
author_facet Di Luca, Andrea
Ostrowska, Barbara
Lorenzo-Moldero, Ivan
Lepedda, Antonio
Swieszkowski, Wojcech
Van Blitterswijk, Clemens
Moroni, Lorenzo
author_sort Di Luca, Andrea
collection PubMed
description Small fractures in bone tissue can heal by themselves, but in case of larger defects current therapies are not completely successful due to several drawbacks. A possible strategy relies on the combination of additive manufactured polymeric scaffolds and human mesenchymal stromal cells (hMSCs). The architecture of bone tissue is characterized by a structural gradient. Long bones display a structural gradient in the radial direction, while flat bones in the axial direction. Such gradient presents a variation in bone density from the cancellous bone to the cortical bone. Therefore, scaffolds presenting a gradient in porosity could be ideal candidates to improve bone tissue regeneration. In this study, we present a construct with a discrete gradient in pore size and characterize its ability to further support the osteogenic differentiation of hMSCs. Furthermore, we studied the behaviour of hMSCs within the different compartments of the gradient scaffolds, showing a correlation between osteogenic differentiation and ECM mineralization, and pore dimensions. Alkaline phosphatase activity and calcium content increased with increasing pore dimensions. Our results indicate that designing structural porosity gradients may be an appealing strategy to support gradual osteogenic differentiation of adult stem cells.
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spelling pubmed-47906312016-03-16 Gradients in pore size enhance the osteogenic differentiation of human mesenchymal stromal cells in three-dimensional scaffolds Di Luca, Andrea Ostrowska, Barbara Lorenzo-Moldero, Ivan Lepedda, Antonio Swieszkowski, Wojcech Van Blitterswijk, Clemens Moroni, Lorenzo Sci Rep Article Small fractures in bone tissue can heal by themselves, but in case of larger defects current therapies are not completely successful due to several drawbacks. A possible strategy relies on the combination of additive manufactured polymeric scaffolds and human mesenchymal stromal cells (hMSCs). The architecture of bone tissue is characterized by a structural gradient. Long bones display a structural gradient in the radial direction, while flat bones in the axial direction. Such gradient presents a variation in bone density from the cancellous bone to the cortical bone. Therefore, scaffolds presenting a gradient in porosity could be ideal candidates to improve bone tissue regeneration. In this study, we present a construct with a discrete gradient in pore size and characterize its ability to further support the osteogenic differentiation of hMSCs. Furthermore, we studied the behaviour of hMSCs within the different compartments of the gradient scaffolds, showing a correlation between osteogenic differentiation and ECM mineralization, and pore dimensions. Alkaline phosphatase activity and calcium content increased with increasing pore dimensions. Our results indicate that designing structural porosity gradients may be an appealing strategy to support gradual osteogenic differentiation of adult stem cells. Nature Publishing Group 2016-03-10 /pmc/articles/PMC4790631/ /pubmed/26961859 http://dx.doi.org/10.1038/srep22898 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Di Luca, Andrea
Ostrowska, Barbara
Lorenzo-Moldero, Ivan
Lepedda, Antonio
Swieszkowski, Wojcech
Van Blitterswijk, Clemens
Moroni, Lorenzo
Gradients in pore size enhance the osteogenic differentiation of human mesenchymal stromal cells in three-dimensional scaffolds
title Gradients in pore size enhance the osteogenic differentiation of human mesenchymal stromal cells in three-dimensional scaffolds
title_full Gradients in pore size enhance the osteogenic differentiation of human mesenchymal stromal cells in three-dimensional scaffolds
title_fullStr Gradients in pore size enhance the osteogenic differentiation of human mesenchymal stromal cells in three-dimensional scaffolds
title_full_unstemmed Gradients in pore size enhance the osteogenic differentiation of human mesenchymal stromal cells in three-dimensional scaffolds
title_short Gradients in pore size enhance the osteogenic differentiation of human mesenchymal stromal cells in three-dimensional scaffolds
title_sort gradients in pore size enhance the osteogenic differentiation of human mesenchymal stromal cells in three-dimensional scaffolds
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4790631/
https://www.ncbi.nlm.nih.gov/pubmed/26961859
http://dx.doi.org/10.1038/srep22898
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