Cargando…
Impact of 4-epi-oxytetracycline on the gut microbiota and blood metabolomics of Wistar rats
The impact of 4-epi-oxytetracycline (4-EOTC), one of the main oxytetracycline (OTC) metabolites, on the gut microbiota and physiological metabolism of Wistar rats was analyzed to explore the dynamic alterations apparent after repeated oral exposure (0.5, 5.0 or 50.0 mg/kg bw) for 15 days as shown by...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4791543/ https://www.ncbi.nlm.nih.gov/pubmed/26976662 http://dx.doi.org/10.1038/srep23141 |
_version_ | 1782421110421192704 |
---|---|
author | Han, Hongxing Xiao, Hailong Zhang, Kai Lu, Zhenmei |
author_facet | Han, Hongxing Xiao, Hailong Zhang, Kai Lu, Zhenmei |
author_sort | Han, Hongxing |
collection | PubMed |
description | The impact of 4-epi-oxytetracycline (4-EOTC), one of the main oxytetracycline (OTC) metabolites, on the gut microbiota and physiological metabolism of Wistar rats was analyzed to explore the dynamic alterations apparent after repeated oral exposure (0.5, 5.0 or 50.0 mg/kg bw) for 15 days as shown by 16S rRNA pyrosequencing and UPLC-Q-TOF/MS analysis. Both principal component analysis and cluster analysis showed consistently altered patterns with distinct differences in the treated groups versus the control groups. 4-EOTC treatment at 5.0 or 50.0 mg/kg increased the relative abundance of the Actinobacteria, specifically Bifidobacteriaceae, and improved the synthesis of lysophosphatidylcholine (LysoPC), as shown by the lipid biomarkers LysoPC(16:0), LysoPC(18:3), LysoPC(20:3), and LysoPC(20:4). The metabolomic analysis of urine samples also identified four other decreased metabolites: diacylglycerol, sphingomyelin, triacylglycerol, and phosphatidylglycerol. Notably, the significant changes observed in these biomarkers demonstrated the ongoing disorder induced by 4-EOTC. Blood and urine analysis revealed that residual 4-EOTC accumulated in the rats, even two weeks after oral 4-EOTC administration, ceased. Thus, through thorough analysis, it can be concluded that the alteration of the gut microbiota and disorders in blood metabolomics are correlated with 4-EOTC treatment. |
format | Online Article Text |
id | pubmed-4791543 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-47915432016-03-16 Impact of 4-epi-oxytetracycline on the gut microbiota and blood metabolomics of Wistar rats Han, Hongxing Xiao, Hailong Zhang, Kai Lu, Zhenmei Sci Rep Article The impact of 4-epi-oxytetracycline (4-EOTC), one of the main oxytetracycline (OTC) metabolites, on the gut microbiota and physiological metabolism of Wistar rats was analyzed to explore the dynamic alterations apparent after repeated oral exposure (0.5, 5.0 or 50.0 mg/kg bw) for 15 days as shown by 16S rRNA pyrosequencing and UPLC-Q-TOF/MS analysis. Both principal component analysis and cluster analysis showed consistently altered patterns with distinct differences in the treated groups versus the control groups. 4-EOTC treatment at 5.0 or 50.0 mg/kg increased the relative abundance of the Actinobacteria, specifically Bifidobacteriaceae, and improved the synthesis of lysophosphatidylcholine (LysoPC), as shown by the lipid biomarkers LysoPC(16:0), LysoPC(18:3), LysoPC(20:3), and LysoPC(20:4). The metabolomic analysis of urine samples also identified four other decreased metabolites: diacylglycerol, sphingomyelin, triacylglycerol, and phosphatidylglycerol. Notably, the significant changes observed in these biomarkers demonstrated the ongoing disorder induced by 4-EOTC. Blood and urine analysis revealed that residual 4-EOTC accumulated in the rats, even two weeks after oral 4-EOTC administration, ceased. Thus, through thorough analysis, it can be concluded that the alteration of the gut microbiota and disorders in blood metabolomics are correlated with 4-EOTC treatment. Nature Publishing Group 2016-03-15 /pmc/articles/PMC4791543/ /pubmed/26976662 http://dx.doi.org/10.1038/srep23141 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Han, Hongxing Xiao, Hailong Zhang, Kai Lu, Zhenmei Impact of 4-epi-oxytetracycline on the gut microbiota and blood metabolomics of Wistar rats |
title | Impact of 4-epi-oxytetracycline on the gut microbiota and blood metabolomics of Wistar rats |
title_full | Impact of 4-epi-oxytetracycline on the gut microbiota and blood metabolomics of Wistar rats |
title_fullStr | Impact of 4-epi-oxytetracycline on the gut microbiota and blood metabolomics of Wistar rats |
title_full_unstemmed | Impact of 4-epi-oxytetracycline on the gut microbiota and blood metabolomics of Wistar rats |
title_short | Impact of 4-epi-oxytetracycline on the gut microbiota and blood metabolomics of Wistar rats |
title_sort | impact of 4-epi-oxytetracycline on the gut microbiota and blood metabolomics of wistar rats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4791543/ https://www.ncbi.nlm.nih.gov/pubmed/26976662 http://dx.doi.org/10.1038/srep23141 |
work_keys_str_mv | AT hanhongxing impactof4epioxytetracyclineonthegutmicrobiotaandbloodmetabolomicsofwistarrats AT xiaohailong impactof4epioxytetracyclineonthegutmicrobiotaandbloodmetabolomicsofwistarrats AT zhangkai impactof4epioxytetracyclineonthegutmicrobiotaandbloodmetabolomicsofwistarrats AT luzhenmei impactof4epioxytetracyclineonthegutmicrobiotaandbloodmetabolomicsofwistarrats |