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siRNA knockdown validation 101: Incorporating negative controls in antibody research
More antibody validation protocols would identify non-specific reagents – a major source of irreproducible research – with the inclusion of negative controls. This article presents an overview of one such method: siRNA knockdown. The authors outline a general protocol, the knockdown mechanism, and t...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
F1000Research
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4792204/ https://www.ncbi.nlm.nih.gov/pubmed/26998240 http://dx.doi.org/10.12688/f1000research.8159.1 |