siRNA knockdown validation 101: Incorporating negative controls in antibody research

More antibody validation protocols would identify non-specific reagents – a major source of irreproducible research – with the inclusion of negative controls. This article presents an overview of one such method: siRNA knockdown. The authors outline a general protocol, the knockdown mechanism, and t...

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Detalles Bibliográficos
Autores principales: Olds, Will, Li, Jason
Formato: Online Artículo Texto
Lenguaje:English
Publicado: F1000Research 2016
Materias:
Opinion Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4792204/
https://www.ncbi.nlm.nih.gov/pubmed/26998240
http://dx.doi.org/10.12688/f1000research.8159.1
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author Olds, Will
Li, Jason
author_facet Olds, Will
Li, Jason
author_sort Olds, Will
collection PubMed
description More antibody validation protocols would identify non-specific reagents – a major source of irreproducible research – with the inclusion of negative controls. This article presents an overview of one such method: siRNA knockdown. The authors outline a general protocol, the knockdown mechanism, and tips for evaluating knockdown experiments.
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spelling pubmed-47922042016-03-18 siRNA knockdown validation 101: Incorporating negative controls in antibody research Olds, Will Li, Jason F1000Res Opinion Article More antibody validation protocols would identify non-specific reagents – a major source of irreproducible research – with the inclusion of negative controls. This article presents an overview of one such method: siRNA knockdown. The authors outline a general protocol, the knockdown mechanism, and tips for evaluating knockdown experiments. F1000Research 2016-03-09 /pmc/articles/PMC4792204/ /pubmed/26998240 http://dx.doi.org/10.12688/f1000research.8159.1 Text en Copyright: © 2016 Olds W and Li J http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Opinion Article
Olds, Will
Li, Jason
siRNA knockdown validation 101: Incorporating negative controls in antibody research
title siRNA knockdown validation 101: Incorporating negative controls in antibody research
title_full siRNA knockdown validation 101: Incorporating negative controls in antibody research
title_fullStr siRNA knockdown validation 101: Incorporating negative controls in antibody research
title_full_unstemmed siRNA knockdown validation 101: Incorporating negative controls in antibody research
title_short siRNA knockdown validation 101: Incorporating negative controls in antibody research
title_sort sirna knockdown validation 101: incorporating negative controls in antibody research
topic Opinion Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4792204/
https://www.ncbi.nlm.nih.gov/pubmed/26998240
http://dx.doi.org/10.12688/f1000research.8159.1
work_keys_str_mv AT oldswill sirnaknockdownvalidation101incorporatingnegativecontrolsinantibodyresearch
AT lijason sirnaknockdownvalidation101incorporatingnegativecontrolsinantibodyresearch

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