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In-Situ Observation of Membrane Protein Folding during Cell-Free Expression

Proper insertion, folding and assembly of functional proteins in biological membranes are key processes to warrant activity of a living cell. Here, we present a novel approach to trace folding and insertion of a nascent membrane protein leaving the ribosome and penetrating the bilayer. Surface Enhan...

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Detalles Bibliográficos
Autores principales: Baumann, Axel, Kerruth, Silke, Fitter, Jörg, Büldt, Georg, Heberle, Joachim, Schlesinger, Ramona, Ataka, Kenichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4792443/
https://www.ncbi.nlm.nih.gov/pubmed/26978519
http://dx.doi.org/10.1371/journal.pone.0151051