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A Novel Manno-Oligosaccharide Binding Protein Identified in Alkaliphilic Bacillus sp. N16-5 Is Involved in Mannan Utilization

ManH, a novel substrate-binding protein of an ABC transporter, was identified from the mannan utilization gene cluster of Bacillus sp. N16-5. We cloned, overexpressed, and purified ManH and measured its binding affinity to different substrates by isothermal titration calorimetry. ManH binds to manno...

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Detalles Bibliográficos
Autores principales: Song, Yajian, Li, Jinshan, Meng, Shan, Yin, Liang, Xue, Yanfen, Ma, Yanhe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4792470/
https://www.ncbi.nlm.nih.gov/pubmed/26978267
http://dx.doi.org/10.1371/journal.pone.0150059