A Novel Manno-Oligosaccharide Binding Protein Identified in Alkaliphilic Bacillus sp. N16-5 Is Involved in Mannan Utilization

ManH, a novel substrate-binding protein of an ABC transporter, was identified from the mannan utilization gene cluster of Bacillus sp. N16-5. We cloned, overexpressed, and purified ManH and measured its binding affinity to different substrates by isothermal titration calorimetry. ManH binds to manno...

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Detalles Bibliográficos
Autores principales: Song, Yajian, Li, Jinshan, Meng, Shan, Yin, Liang, Xue, Yanfen, Ma, Yanhe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4792470/
https://www.ncbi.nlm.nih.gov/pubmed/26978267
http://dx.doi.org/10.1371/journal.pone.0150059
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author Song, Yajian
Li, Jinshan
Meng, Shan
Yin, Liang
Xue, Yanfen
Ma, Yanhe
author_facet Song, Yajian
Li, Jinshan
Meng, Shan
Yin, Liang
Xue, Yanfen
Ma, Yanhe
author_sort Song, Yajian
collection PubMed
description ManH, a novel substrate-binding protein of an ABC transporter, was identified from the mannan utilization gene cluster of Bacillus sp. N16-5. We cloned, overexpressed, and purified ManH and measured its binding affinity to different substrates by isothermal titration calorimetry. ManH binds to mannotriose, mannotetraose, mannopentose, and galactosyl-mannotriose with dissociation constants in the micromolar range. Deletion of manH led to decreased growth ability of the strain when cultivated in medium with manno-oligosaccharides or mannan as the carbon source. ManH belongs to a manno-oligosaccharide transporter and plays an important role in mannan utilization by Bacillus sp. N16-5.
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spelling pubmed-47924702016-03-23 A Novel Manno-Oligosaccharide Binding Protein Identified in Alkaliphilic Bacillus sp. N16-5 Is Involved in Mannan Utilization Song, Yajian Li, Jinshan Meng, Shan Yin, Liang Xue, Yanfen Ma, Yanhe PLoS One Research Article ManH, a novel substrate-binding protein of an ABC transporter, was identified from the mannan utilization gene cluster of Bacillus sp. N16-5. We cloned, overexpressed, and purified ManH and measured its binding affinity to different substrates by isothermal titration calorimetry. ManH binds to mannotriose, mannotetraose, mannopentose, and galactosyl-mannotriose with dissociation constants in the micromolar range. Deletion of manH led to decreased growth ability of the strain when cultivated in medium with manno-oligosaccharides or mannan as the carbon source. ManH belongs to a manno-oligosaccharide transporter and plays an important role in mannan utilization by Bacillus sp. N16-5. Public Library of Science 2016-03-15 /pmc/articles/PMC4792470/ /pubmed/26978267 http://dx.doi.org/10.1371/journal.pone.0150059 Text en © 2016 Song et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Song, Yajian
Li, Jinshan
Meng, Shan
Yin, Liang
Xue, Yanfen
Ma, Yanhe
A Novel Manno-Oligosaccharide Binding Protein Identified in Alkaliphilic Bacillus sp. N16-5 Is Involved in Mannan Utilization
title A Novel Manno-Oligosaccharide Binding Protein Identified in Alkaliphilic Bacillus sp. N16-5 Is Involved in Mannan Utilization
title_full A Novel Manno-Oligosaccharide Binding Protein Identified in Alkaliphilic Bacillus sp. N16-5 Is Involved in Mannan Utilization
title_fullStr A Novel Manno-Oligosaccharide Binding Protein Identified in Alkaliphilic Bacillus sp. N16-5 Is Involved in Mannan Utilization
title_full_unstemmed A Novel Manno-Oligosaccharide Binding Protein Identified in Alkaliphilic Bacillus sp. N16-5 Is Involved in Mannan Utilization
title_short A Novel Manno-Oligosaccharide Binding Protein Identified in Alkaliphilic Bacillus sp. N16-5 Is Involved in Mannan Utilization
title_sort novel manno-oligosaccharide binding protein identified in alkaliphilic bacillus sp. n16-5 is involved in mannan utilization
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4792470/
https://www.ncbi.nlm.nih.gov/pubmed/26978267
http://dx.doi.org/10.1371/journal.pone.0150059
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