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The Rtr1p CTD phosphatase autoregulates its mRNA through a degradation pathway involving the REX exonucleases

Rtr1p is a phosphatase that impacts gene expression by modulating the phosphorylation status of the C-terminal domain of the large subunit of RNA polymerase II. Here, we show that Rtr1p is a component of a novel mRNA degradation pathway that promotes its autoregulation through turnover of its own mR...

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Detalles Bibliográficos
Autores principales: Hodko, Domagoj, Ward, Taylor, Chanfreau, Guillaume
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4793211/
https://www.ncbi.nlm.nih.gov/pubmed/26843527
http://dx.doi.org/10.1261/rna.055723.115
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author Hodko, Domagoj
Ward, Taylor
Chanfreau, Guillaume
author_facet Hodko, Domagoj
Ward, Taylor
Chanfreau, Guillaume
author_sort Hodko, Domagoj
collection PubMed
description Rtr1p is a phosphatase that impacts gene expression by modulating the phosphorylation status of the C-terminal domain of the large subunit of RNA polymerase II. Here, we show that Rtr1p is a component of a novel mRNA degradation pathway that promotes its autoregulation through turnover of its own mRNA. We show that the 3′UTR of the RTR1 mRNA contains a cis element that destabilizes this mRNA. RTR1 mRNA turnover is achieved through binding of Rtr1p to the RTR1 mRNP in a manner that is dependent on this cis element. Genetic evidence shows that Rtr1p-mediated decay of the RTR1 mRNA involves the 5′-3′ DExD/H-box RNA helicase Dhh1p and the 3′-5′ exonucleases Rex2p and Rex3p. Rtr1p and Rex3p are found associated with Dhh1p, suggesting a model for recruiting the REX exonucleases to the RTR1 mRNA for degradation. Rtr1p-mediated decay potentially impacts additional transcripts, including the unspliced BMH2 pre-mRNA. We propose that Rtr1p may imprint its RNA targets cotranscriptionally and determine their downstream degradation mechanism by directing these transcripts to a novel turnover pathway that involves Rtr1p, Dhh1p, and the REX family of exonucleases.
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spelling pubmed-47932112016-04-01 The Rtr1p CTD phosphatase autoregulates its mRNA through a degradation pathway involving the REX exonucleases Hodko, Domagoj Ward, Taylor Chanfreau, Guillaume RNA Article Rtr1p is a phosphatase that impacts gene expression by modulating the phosphorylation status of the C-terminal domain of the large subunit of RNA polymerase II. Here, we show that Rtr1p is a component of a novel mRNA degradation pathway that promotes its autoregulation through turnover of its own mRNA. We show that the 3′UTR of the RTR1 mRNA contains a cis element that destabilizes this mRNA. RTR1 mRNA turnover is achieved through binding of Rtr1p to the RTR1 mRNP in a manner that is dependent on this cis element. Genetic evidence shows that Rtr1p-mediated decay of the RTR1 mRNA involves the 5′-3′ DExD/H-box RNA helicase Dhh1p and the 3′-5′ exonucleases Rex2p and Rex3p. Rtr1p and Rex3p are found associated with Dhh1p, suggesting a model for recruiting the REX exonucleases to the RTR1 mRNA for degradation. Rtr1p-mediated decay potentially impacts additional transcripts, including the unspliced BMH2 pre-mRNA. We propose that Rtr1p may imprint its RNA targets cotranscriptionally and determine their downstream degradation mechanism by directing these transcripts to a novel turnover pathway that involves Rtr1p, Dhh1p, and the REX family of exonucleases. Cold Spring Harbor Laboratory Press 2016-04 /pmc/articles/PMC4793211/ /pubmed/26843527 http://dx.doi.org/10.1261/rna.055723.115 Text en © 2016 Hodko et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society http://creativecommons.org/licenses/by/4.0/ This article, published in RNA, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Hodko, Domagoj
Ward, Taylor
Chanfreau, Guillaume
The Rtr1p CTD phosphatase autoregulates its mRNA through a degradation pathway involving the REX exonucleases
title The Rtr1p CTD phosphatase autoregulates its mRNA through a degradation pathway involving the REX exonucleases
title_full The Rtr1p CTD phosphatase autoregulates its mRNA through a degradation pathway involving the REX exonucleases
title_fullStr The Rtr1p CTD phosphatase autoregulates its mRNA through a degradation pathway involving the REX exonucleases
title_full_unstemmed The Rtr1p CTD phosphatase autoregulates its mRNA through a degradation pathway involving the REX exonucleases
title_short The Rtr1p CTD phosphatase autoregulates its mRNA through a degradation pathway involving the REX exonucleases
title_sort rtr1p ctd phosphatase autoregulates its mrna through a degradation pathway involving the rex exonucleases
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4793211/
https://www.ncbi.nlm.nih.gov/pubmed/26843527
http://dx.doi.org/10.1261/rna.055723.115
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