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The Rtr1p CTD phosphatase autoregulates its mRNA through a degradation pathway involving the REX exonucleases
Rtr1p is a phosphatase that impacts gene expression by modulating the phosphorylation status of the C-terminal domain of the large subunit of RNA polymerase II. Here, we show that Rtr1p is a component of a novel mRNA degradation pathway that promotes its autoregulation through turnover of its own mR...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4793211/ https://www.ncbi.nlm.nih.gov/pubmed/26843527 http://dx.doi.org/10.1261/rna.055723.115 |
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author | Hodko, Domagoj Ward, Taylor Chanfreau, Guillaume |
author_facet | Hodko, Domagoj Ward, Taylor Chanfreau, Guillaume |
author_sort | Hodko, Domagoj |
collection | PubMed |
description | Rtr1p is a phosphatase that impacts gene expression by modulating the phosphorylation status of the C-terminal domain of the large subunit of RNA polymerase II. Here, we show that Rtr1p is a component of a novel mRNA degradation pathway that promotes its autoregulation through turnover of its own mRNA. We show that the 3′UTR of the RTR1 mRNA contains a cis element that destabilizes this mRNA. RTR1 mRNA turnover is achieved through binding of Rtr1p to the RTR1 mRNP in a manner that is dependent on this cis element. Genetic evidence shows that Rtr1p-mediated decay of the RTR1 mRNA involves the 5′-3′ DExD/H-box RNA helicase Dhh1p and the 3′-5′ exonucleases Rex2p and Rex3p. Rtr1p and Rex3p are found associated with Dhh1p, suggesting a model for recruiting the REX exonucleases to the RTR1 mRNA for degradation. Rtr1p-mediated decay potentially impacts additional transcripts, including the unspliced BMH2 pre-mRNA. We propose that Rtr1p may imprint its RNA targets cotranscriptionally and determine their downstream degradation mechanism by directing these transcripts to a novel turnover pathway that involves Rtr1p, Dhh1p, and the REX family of exonucleases. |
format | Online Article Text |
id | pubmed-4793211 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Cold Spring Harbor Laboratory Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-47932112016-04-01 The Rtr1p CTD phosphatase autoregulates its mRNA through a degradation pathway involving the REX exonucleases Hodko, Domagoj Ward, Taylor Chanfreau, Guillaume RNA Article Rtr1p is a phosphatase that impacts gene expression by modulating the phosphorylation status of the C-terminal domain of the large subunit of RNA polymerase II. Here, we show that Rtr1p is a component of a novel mRNA degradation pathway that promotes its autoregulation through turnover of its own mRNA. We show that the 3′UTR of the RTR1 mRNA contains a cis element that destabilizes this mRNA. RTR1 mRNA turnover is achieved through binding of Rtr1p to the RTR1 mRNP in a manner that is dependent on this cis element. Genetic evidence shows that Rtr1p-mediated decay of the RTR1 mRNA involves the 5′-3′ DExD/H-box RNA helicase Dhh1p and the 3′-5′ exonucleases Rex2p and Rex3p. Rtr1p and Rex3p are found associated with Dhh1p, suggesting a model for recruiting the REX exonucleases to the RTR1 mRNA for degradation. Rtr1p-mediated decay potentially impacts additional transcripts, including the unspliced BMH2 pre-mRNA. We propose that Rtr1p may imprint its RNA targets cotranscriptionally and determine their downstream degradation mechanism by directing these transcripts to a novel turnover pathway that involves Rtr1p, Dhh1p, and the REX family of exonucleases. Cold Spring Harbor Laboratory Press 2016-04 /pmc/articles/PMC4793211/ /pubmed/26843527 http://dx.doi.org/10.1261/rna.055723.115 Text en © 2016 Hodko et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society http://creativecommons.org/licenses/by/4.0/ This article, published in RNA, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Hodko, Domagoj Ward, Taylor Chanfreau, Guillaume The Rtr1p CTD phosphatase autoregulates its mRNA through a degradation pathway involving the REX exonucleases |
title | The Rtr1p CTD phosphatase autoregulates its mRNA through a degradation pathway involving the REX exonucleases |
title_full | The Rtr1p CTD phosphatase autoregulates its mRNA through a degradation pathway involving the REX exonucleases |
title_fullStr | The Rtr1p CTD phosphatase autoregulates its mRNA through a degradation pathway involving the REX exonucleases |
title_full_unstemmed | The Rtr1p CTD phosphatase autoregulates its mRNA through a degradation pathway involving the REX exonucleases |
title_short | The Rtr1p CTD phosphatase autoregulates its mRNA through a degradation pathway involving the REX exonucleases |
title_sort | rtr1p ctd phosphatase autoregulates its mrna through a degradation pathway involving the rex exonucleases |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4793211/ https://www.ncbi.nlm.nih.gov/pubmed/26843527 http://dx.doi.org/10.1261/rna.055723.115 |
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