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The in vivo dynamics of TCERG1, a factor that couples transcriptional elongation with splicing
Coupling between transcription and RNA processing is key for gene regulation. Using live-cell photobleaching techniques, we investigated the factor TCERG1, which coordinates transcriptional elongation with splicing. We demonstrate that TCERG1 is highly mobile in the nucleoplasm and that this mobilit...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4793212/ https://www.ncbi.nlm.nih.gov/pubmed/26873599 http://dx.doi.org/10.1261/rna.052795.115 |
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author | Sánchez-Hernández, Noemí Boireau, Stéphanie Schmidt, Ute Muñoz-Cobo, Juan Pablo Hernández-Munain, Cristina Bertrand, Edouard Suñé, Carlos |
author_facet | Sánchez-Hernández, Noemí Boireau, Stéphanie Schmidt, Ute Muñoz-Cobo, Juan Pablo Hernández-Munain, Cristina Bertrand, Edouard Suñé, Carlos |
author_sort | Sánchez-Hernández, Noemí |
collection | PubMed |
description | Coupling between transcription and RNA processing is key for gene regulation. Using live-cell photobleaching techniques, we investigated the factor TCERG1, which coordinates transcriptional elongation with splicing. We demonstrate that TCERG1 is highly mobile in the nucleoplasm and that this mobility is slightly decreased when it is associated with speckles. Dichloro-1-β-D-ribofuranosylbenzimidazole (DRB) but not α-amanitin treatment reduced the mobility of TCERG1, which suggests interaction with paused transcription elongation complexes. We found that TCERG1 mobility is rapid at the transcription site (TS) of a reporter that splices post-transcriptionally and that TCERG1 is recruited to the active TS independent of the CTD of RNAPII, thus excluding phosphorylated CTD as a requirement for recruiting this factor to the TS. Importantly, the mobility of TCERG1 is reduced when the reporter splices cotranscriptionally, which suggests that TCERG1 forms new macromolecular complexes when splicing occurs cotranscriptionally. In this condition, spliceostatin A has no effect, indicating that TCERG1 rapidly binds and dissociates from stalled spliceosomal complexes and that the mobility properties of TCERG1 do not depend on events occurring after the initial spliceosome formation. Taken together, these data suggest that TCERG1 binds independently to elongation and splicing complexes, thus performing their coupling by transient interactions rather than by stable association with one or the other complexes. This finding has conceptual implications for understanding the coupling between transcription and RNA processing. |
format | Online Article Text |
id | pubmed-4793212 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Cold Spring Harbor Laboratory Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-47932122017-04-01 The in vivo dynamics of TCERG1, a factor that couples transcriptional elongation with splicing Sánchez-Hernández, Noemí Boireau, Stéphanie Schmidt, Ute Muñoz-Cobo, Juan Pablo Hernández-Munain, Cristina Bertrand, Edouard Suñé, Carlos RNA Article Coupling between transcription and RNA processing is key for gene regulation. Using live-cell photobleaching techniques, we investigated the factor TCERG1, which coordinates transcriptional elongation with splicing. We demonstrate that TCERG1 is highly mobile in the nucleoplasm and that this mobility is slightly decreased when it is associated with speckles. Dichloro-1-β-D-ribofuranosylbenzimidazole (DRB) but not α-amanitin treatment reduced the mobility of TCERG1, which suggests interaction with paused transcription elongation complexes. We found that TCERG1 mobility is rapid at the transcription site (TS) of a reporter that splices post-transcriptionally and that TCERG1 is recruited to the active TS independent of the CTD of RNAPII, thus excluding phosphorylated CTD as a requirement for recruiting this factor to the TS. Importantly, the mobility of TCERG1 is reduced when the reporter splices cotranscriptionally, which suggests that TCERG1 forms new macromolecular complexes when splicing occurs cotranscriptionally. In this condition, spliceostatin A has no effect, indicating that TCERG1 rapidly binds and dissociates from stalled spliceosomal complexes and that the mobility properties of TCERG1 do not depend on events occurring after the initial spliceosome formation. Taken together, these data suggest that TCERG1 binds independently to elongation and splicing complexes, thus performing their coupling by transient interactions rather than by stable association with one or the other complexes. This finding has conceptual implications for understanding the coupling between transcription and RNA processing. Cold Spring Harbor Laboratory Press 2016-04 /pmc/articles/PMC4793212/ /pubmed/26873599 http://dx.doi.org/10.1261/rna.052795.115 Text en © 2016 Sánchez-Hernández et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/. |
spellingShingle | Article Sánchez-Hernández, Noemí Boireau, Stéphanie Schmidt, Ute Muñoz-Cobo, Juan Pablo Hernández-Munain, Cristina Bertrand, Edouard Suñé, Carlos The in vivo dynamics of TCERG1, a factor that couples transcriptional elongation with splicing |
title | The in vivo dynamics of TCERG1, a factor that couples transcriptional elongation with splicing |
title_full | The in vivo dynamics of TCERG1, a factor that couples transcriptional elongation with splicing |
title_fullStr | The in vivo dynamics of TCERG1, a factor that couples transcriptional elongation with splicing |
title_full_unstemmed | The in vivo dynamics of TCERG1, a factor that couples transcriptional elongation with splicing |
title_short | The in vivo dynamics of TCERG1, a factor that couples transcriptional elongation with splicing |
title_sort | in vivo dynamics of tcerg1, a factor that couples transcriptional elongation with splicing |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4793212/ https://www.ncbi.nlm.nih.gov/pubmed/26873599 http://dx.doi.org/10.1261/rna.052795.115 |
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