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A reliable method for quantification of splice variants using RT-qPCR
BACKGROUND: The majority of protein isoforms arise from alternative splicing of the encoding primary RNA transcripts. To understand the significance of single splicing events, reliable techniques are needed to determine their incidence. However, existing methods are labour-intensive, error-prone or...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4793508/ https://www.ncbi.nlm.nih.gov/pubmed/26979160 http://dx.doi.org/10.1186/s12867-016-0060-1 |