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A reliable method for quantification of splice variants using RT-qPCR

BACKGROUND: The majority of protein isoforms arise from alternative splicing of the encoding primary RNA transcripts. To understand the significance of single splicing events, reliable techniques are needed to determine their incidence. However, existing methods are labour-intensive, error-prone or...

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Detalles Bibliográficos
Autores principales: Camacho Londoño, Julia, Philipp, Stephan E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4793508/
https://www.ncbi.nlm.nih.gov/pubmed/26979160
http://dx.doi.org/10.1186/s12867-016-0060-1