Improved Production of Active Streptomyces griseus Trypsin with a Novel Auto-Catalyzed Strategy

N-terminal sequences play crucial roles in regulating expression, translation, activation and enzymatic properties of proteins. To reduce cell toxicity of intracellular trypsin and increase secretory expression, we developed a novel auto-catalyzed strategy to produce recombinant trypsin by engineeri...

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Detalles Bibliográficos
Autores principales: Zhang, Yunfeng, Ling, Zhenmin, Du, Guocheng, Chen, Jian, Kang, Zhen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4794721/
https://www.ncbi.nlm.nih.gov/pubmed/26983398
http://dx.doi.org/10.1038/srep23158

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4794721/
https://www.ncbi.nlm.nih.gov/pubmed/26983398
http://dx.doi.org/10.1038/srep23158

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