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Improved Production of Active Streptomyces griseus Trypsin with a Novel Auto-Catalyzed Strategy
N-terminal sequences play crucial roles in regulating expression, translation, activation and enzymatic properties of proteins. To reduce cell toxicity of intracellular trypsin and increase secretory expression, we developed a novel auto-catalyzed strategy to produce recombinant trypsin by engineeri...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4794721/ https://www.ncbi.nlm.nih.gov/pubmed/26983398 http://dx.doi.org/10.1038/srep23158 |