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Sequence-Dependent Fluorescence of Cy3- and Cy5-Labeled Double-Stranded DNA

[Image: see text] The fluorescent intensity of Cy3 and Cy5 dyes is strongly dependent on the nucleobase sequence of the labeled oligonucleotides. Sequence-dependent fluorescence may significantly influence the data obtained from many common experimental methods based on fluorescence detection of nuc...

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Detalles Bibliográficos
Autores principales: Kretschy, Nicole, Sack, Matej, Somoza, Mark M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2016
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4796863/
https://www.ncbi.nlm.nih.gov/pubmed/26895222
http://dx.doi.org/10.1021/acs.bioconjchem.6b00053