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Production of unstable proteins through the formation of stable core complexes

Purification of proteins that participate in large transient complexes is impeded by low amounts, heterogeneity, instability and poor solubility. To circumvent these difficulties we set up a methodology that enables the production of stable complexes for structural and functional studies. This proce...

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Detalles Bibliográficos
Autores principales: Levy, Nicolas, Eiler, Sylvia, Pradeau-Aubreton, Karine, Maillot, Benoit, Stricher, François, Ruff, Marc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4800440/
https://www.ncbi.nlm.nih.gov/pubmed/26983699
http://dx.doi.org/10.1038/ncomms10932