Rapid and Robust PCR-Based All-Recombinant Cloning Methodology

We report here a PCR-based cloning methodology that requires no post-PCR modifications such as restriction digestion and phosphorylation of the amplified DNA. The advantage of the present method is that it yields only recombinant clones thus eliminating the need for screening. Two DNA amplification...

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Detalles Bibliográficos
Autores principales: Dubey, Abhishek Anil, Singh, Manika Indrajit, Jain, Vikas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4805250/
https://www.ncbi.nlm.nih.gov/pubmed/27007922
http://dx.doi.org/10.1371/journal.pone.0152106

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4805250/
https://www.ncbi.nlm.nih.gov/pubmed/27007922
http://dx.doi.org/10.1371/journal.pone.0152106

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