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Electrical lysis of cells for detergent-free droplet assays

Efficient lysis is critical when analyzing single cells in microfluidic droplets, but existing methods utilize detergents that can interfere with the assays to be performed. We demonstrate robust cell lysis without the use of detergents or other chemicals. In our method, cells are exposed to electri...

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Detalles Bibliográficos
Autores principales: de Lange, N., Tran, T. M., Abate, A. R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AIP Publishing LLC 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4808063/
https://www.ncbi.nlm.nih.gov/pubmed/27051471
http://dx.doi.org/10.1063/1.4944742