Combined DECS Analysis and Next-Generation Sequencing Enable Efficient Detection of Novel Plant RNA Viruses

The presence of high molecular weight double-stranded RNA (dsRNA) within plant cells is an indicator of infection with RNA viruses as these possess genomic or replicative dsRNA. DECS (dsRNA isolation, exhaustive amplification, cloning, and sequencing) analysis has been shown to be capable of detecti...

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Detalles Bibliográficos
Autores principales: Yanagisawa, Hironobu, Tomita, Reiko, Katsu, Koji, Uehara, Takuya, Atsumi, Go, Tateda, Chika, Kobayashi, Kappei, Sekine, Ken-Taro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4810260/
https://www.ncbi.nlm.nih.gov/pubmed/27072419
http://dx.doi.org/10.3390/v8030070

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4810260/
https://www.ncbi.nlm.nih.gov/pubmed/27072419
http://dx.doi.org/10.3390/v8030070

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