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Combined DECS Analysis and Next-Generation Sequencing Enable Efficient Detection of Novel Plant RNA Viruses
The presence of high molecular weight double-stranded RNA (dsRNA) within plant cells is an indicator of infection with RNA viruses as these possess genomic or replicative dsRNA. DECS (dsRNA isolation, exhaustive amplification, cloning, and sequencing) analysis has been shown to be capable of detecti...
Autores principales: | Yanagisawa, Hironobu, Tomita, Reiko, Katsu, Koji, Uehara, Takuya, Atsumi, Go, Tateda, Chika, Kobayashi, Kappei, Sekine, Ken-Taro |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4810260/ https://www.ncbi.nlm.nih.gov/pubmed/27072419 http://dx.doi.org/10.3390/v8030070 |
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