Cargando…
Adiponectin at Physiologically Relevant Concentrations Enhances the Vasorelaxative Effect of Acetylcholine via Cav-1/AdipoR-1 Signaling
Clinical studies have identified hypoadiponectinemia as an independent hypertension risk factor. It is known that adiponectin (APN) can directly cause vasodilation, but the doses required exceed physiologic levels several fold. In the current study, we determine the effect of physiologically relevan...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4811582/ https://www.ncbi.nlm.nih.gov/pubmed/27023866 http://dx.doi.org/10.1371/journal.pone.0152247 |
_version_ | 1782423999021580288 |
---|---|
author | Du, Yunhui Li, Rui Lau, Wayne Bigond Zhao, Jianli Lopez, Bernard Christopher, Theodore A. Ma, Xin-Liang Wang, Yajing |
author_facet | Du, Yunhui Li, Rui Lau, Wayne Bigond Zhao, Jianli Lopez, Bernard Christopher, Theodore A. Ma, Xin-Liang Wang, Yajing |
author_sort | Du, Yunhui |
collection | PubMed |
description | Clinical studies have identified hypoadiponectinemia as an independent hypertension risk factor. It is known that adiponectin (APN) can directly cause vasodilation, but the doses required exceed physiologic levels several fold. In the current study, we determine the effect of physiologically relevant APN concentrations upon vascular tone, and investigate the mechanism(s) responsible. Physiologic APN concentrations alone induced no significant vasorelaxation. Interestingly, pretreatment of wild type mouse aortae with physiologic APN levels significantly enhanced acetylcholine (ACh)-induced vasorelaxation (P<0.01), an endothelium-dependent and nitric oxide (NO)-mediated process. Knockout of adiponectin receptor 1 (AdipoR1) or caveolin-1 (Cav-1, a cell signaling facilitating molecule), but not adiponectin receptor 2 (AdipoR2) abolished APN-enhanced ACh-induced vasorelaxation. Immunoblot assay revealed APN promoted the AdipoR1/Cav1 signaling complex in human endothelial cells. Treatment of HUVECs with physiologic APN concentrations caused significant eNOS phosphorylation and nitric oxide (NO) production (P<0.01), an effect abolished in knockdown of either AdipoR1 or Cav-1. Taken together, these data demonstrate for the first time physiologic APN levels enhance the vasorelaxative response to ACh by inducing NO production through AdipoR1/Cav-1 mediated signaling. In physiologic conditions, APN plays an important function of maintaining vascular tone. |
format | Online Article Text |
id | pubmed-4811582 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-48115822016-04-05 Adiponectin at Physiologically Relevant Concentrations Enhances the Vasorelaxative Effect of Acetylcholine via Cav-1/AdipoR-1 Signaling Du, Yunhui Li, Rui Lau, Wayne Bigond Zhao, Jianli Lopez, Bernard Christopher, Theodore A. Ma, Xin-Liang Wang, Yajing PLoS One Research Article Clinical studies have identified hypoadiponectinemia as an independent hypertension risk factor. It is known that adiponectin (APN) can directly cause vasodilation, but the doses required exceed physiologic levels several fold. In the current study, we determine the effect of physiologically relevant APN concentrations upon vascular tone, and investigate the mechanism(s) responsible. Physiologic APN concentrations alone induced no significant vasorelaxation. Interestingly, pretreatment of wild type mouse aortae with physiologic APN levels significantly enhanced acetylcholine (ACh)-induced vasorelaxation (P<0.01), an endothelium-dependent and nitric oxide (NO)-mediated process. Knockout of adiponectin receptor 1 (AdipoR1) or caveolin-1 (Cav-1, a cell signaling facilitating molecule), but not adiponectin receptor 2 (AdipoR2) abolished APN-enhanced ACh-induced vasorelaxation. Immunoblot assay revealed APN promoted the AdipoR1/Cav1 signaling complex in human endothelial cells. Treatment of HUVECs with physiologic APN concentrations caused significant eNOS phosphorylation and nitric oxide (NO) production (P<0.01), an effect abolished in knockdown of either AdipoR1 or Cav-1. Taken together, these data demonstrate for the first time physiologic APN levels enhance the vasorelaxative response to ACh by inducing NO production through AdipoR1/Cav-1 mediated signaling. In physiologic conditions, APN plays an important function of maintaining vascular tone. Public Library of Science 2016-03-29 /pmc/articles/PMC4811582/ /pubmed/27023866 http://dx.doi.org/10.1371/journal.pone.0152247 Text en © 2016 Du et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Du, Yunhui Li, Rui Lau, Wayne Bigond Zhao, Jianli Lopez, Bernard Christopher, Theodore A. Ma, Xin-Liang Wang, Yajing Adiponectin at Physiologically Relevant Concentrations Enhances the Vasorelaxative Effect of Acetylcholine via Cav-1/AdipoR-1 Signaling |
title | Adiponectin at Physiologically Relevant Concentrations Enhances the Vasorelaxative Effect of Acetylcholine via Cav-1/AdipoR-1 Signaling |
title_full | Adiponectin at Physiologically Relevant Concentrations Enhances the Vasorelaxative Effect of Acetylcholine via Cav-1/AdipoR-1 Signaling |
title_fullStr | Adiponectin at Physiologically Relevant Concentrations Enhances the Vasorelaxative Effect of Acetylcholine via Cav-1/AdipoR-1 Signaling |
title_full_unstemmed | Adiponectin at Physiologically Relevant Concentrations Enhances the Vasorelaxative Effect of Acetylcholine via Cav-1/AdipoR-1 Signaling |
title_short | Adiponectin at Physiologically Relevant Concentrations Enhances the Vasorelaxative Effect of Acetylcholine via Cav-1/AdipoR-1 Signaling |
title_sort | adiponectin at physiologically relevant concentrations enhances the vasorelaxative effect of acetylcholine via cav-1/adipor-1 signaling |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4811582/ https://www.ncbi.nlm.nih.gov/pubmed/27023866 http://dx.doi.org/10.1371/journal.pone.0152247 |
work_keys_str_mv | AT duyunhui adiponectinatphysiologicallyrelevantconcentrationsenhancesthevasorelaxativeeffectofacetylcholineviacav1adipor1signaling AT lirui adiponectinatphysiologicallyrelevantconcentrationsenhancesthevasorelaxativeeffectofacetylcholineviacav1adipor1signaling AT lauwaynebigond adiponectinatphysiologicallyrelevantconcentrationsenhancesthevasorelaxativeeffectofacetylcholineviacav1adipor1signaling AT zhaojianli adiponectinatphysiologicallyrelevantconcentrationsenhancesthevasorelaxativeeffectofacetylcholineviacav1adipor1signaling AT lopezbernard adiponectinatphysiologicallyrelevantconcentrationsenhancesthevasorelaxativeeffectofacetylcholineviacav1adipor1signaling AT christophertheodorea adiponectinatphysiologicallyrelevantconcentrationsenhancesthevasorelaxativeeffectofacetylcholineviacav1adipor1signaling AT maxinliang adiponectinatphysiologicallyrelevantconcentrationsenhancesthevasorelaxativeeffectofacetylcholineviacav1adipor1signaling AT wangyajing adiponectinatphysiologicallyrelevantconcentrationsenhancesthevasorelaxativeeffectofacetylcholineviacav1adipor1signaling |