Structural Elucidation and Toxicity Assessment of Degraded Products of Aflatoxin B1 and B2 by Aqueous Extracts of Trachyspermum ammi
In this study aqueous extract of seeds and leaves of Trachyspermum ammi were evaluated for their ability to detoxify aflatoxin B1 and B2 (AFB1; 100 μg L(−1) and AFB2; 50 μg L(−1)) by in vitro and in vivo assays. Results indicated that T. ammi seeds extract was found to be significant (P < 0.05) i...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4811950/ https://www.ncbi.nlm.nih.gov/pubmed/27064492 http://dx.doi.org/10.3389/fmicb.2016.00346 |
_version_ | 1782424059209842688 |
---|---|
author | Iram, Wajiha Anjum, Tehmina Iqbal, Mazhar Ghaffar, Abdul Abbas, Mateen |
author_facet | Iram, Wajiha Anjum, Tehmina Iqbal, Mazhar Ghaffar, Abdul Abbas, Mateen |
author_sort | Iram, Wajiha |
collection | PubMed |
description | In this study aqueous extract of seeds and leaves of Trachyspermum ammi were evaluated for their ability to detoxify aflatoxin B1 and B2 (AFB1; 100 μg L(−1) and AFB2; 50 μg L(−1)) by in vitro and in vivo assays. Results indicated that T. ammi seeds extract was found to be significant (P < 0.05) in degrading AFB1 and AFB2 i.e., 92.8 and 91.9% respectively. However, T. ammi leaves extract proved to be less efficient in degrading these aflatoxins, under optimized conditions i.e., pH 8, temperature 30°C and incubation period of 72 h. The structural elucidation of degraded toxin products by LCMS/MS analysis showed that eight degraded products of AFB1 and AFB2 were formed. MS/MS spectra showed that most of the products were formed by the removal of double bond in the terminal furan ring and modification of lactone group indicating less toxicity as compared to parent compounds. Brine shrimps bioassay further confirmed the low toxicity of degraded products, showing that T. ammi seeds extract can be used as an effective tool for the detoxification of aflatoxins. |
format | Online Article Text |
id | pubmed-4811950 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-48119502016-04-08 Structural Elucidation and Toxicity Assessment of Degraded Products of Aflatoxin B1 and B2 by Aqueous Extracts of Trachyspermum ammi Iram, Wajiha Anjum, Tehmina Iqbal, Mazhar Ghaffar, Abdul Abbas, Mateen Front Microbiol Microbiology In this study aqueous extract of seeds and leaves of Trachyspermum ammi were evaluated for their ability to detoxify aflatoxin B1 and B2 (AFB1; 100 μg L(−1) and AFB2; 50 μg L(−1)) by in vitro and in vivo assays. Results indicated that T. ammi seeds extract was found to be significant (P < 0.05) in degrading AFB1 and AFB2 i.e., 92.8 and 91.9% respectively. However, T. ammi leaves extract proved to be less efficient in degrading these aflatoxins, under optimized conditions i.e., pH 8, temperature 30°C and incubation period of 72 h. The structural elucidation of degraded toxin products by LCMS/MS analysis showed that eight degraded products of AFB1 and AFB2 were formed. MS/MS spectra showed that most of the products were formed by the removal of double bond in the terminal furan ring and modification of lactone group indicating less toxicity as compared to parent compounds. Brine shrimps bioassay further confirmed the low toxicity of degraded products, showing that T. ammi seeds extract can be used as an effective tool for the detoxification of aflatoxins. Frontiers Media S.A. 2016-03-30 /pmc/articles/PMC4811950/ /pubmed/27064492 http://dx.doi.org/10.3389/fmicb.2016.00346 Text en Copyright © 2016 Iram, Anjum, Iqbal, Ghaffar and Abbas. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Iram, Wajiha Anjum, Tehmina Iqbal, Mazhar Ghaffar, Abdul Abbas, Mateen Structural Elucidation and Toxicity Assessment of Degraded Products of Aflatoxin B1 and B2 by Aqueous Extracts of Trachyspermum ammi |
title | Structural Elucidation and Toxicity Assessment of Degraded Products of Aflatoxin B1 and B2 by Aqueous Extracts of Trachyspermum ammi |
title_full | Structural Elucidation and Toxicity Assessment of Degraded Products of Aflatoxin B1 and B2 by Aqueous Extracts of Trachyspermum ammi |
title_fullStr | Structural Elucidation and Toxicity Assessment of Degraded Products of Aflatoxin B1 and B2 by Aqueous Extracts of Trachyspermum ammi |
title_full_unstemmed | Structural Elucidation and Toxicity Assessment of Degraded Products of Aflatoxin B1 and B2 by Aqueous Extracts of Trachyspermum ammi |
title_short | Structural Elucidation and Toxicity Assessment of Degraded Products of Aflatoxin B1 and B2 by Aqueous Extracts of Trachyspermum ammi |
title_sort | structural elucidation and toxicity assessment of degraded products of aflatoxin b1 and b2 by aqueous extracts of trachyspermum ammi |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4811950/ https://www.ncbi.nlm.nih.gov/pubmed/27064492 http://dx.doi.org/10.3389/fmicb.2016.00346 |
work_keys_str_mv | AT iramwajiha structuralelucidationandtoxicityassessmentofdegradedproductsofaflatoxinb1andb2byaqueousextractsoftrachyspermumammi AT anjumtehmina structuralelucidationandtoxicityassessmentofdegradedproductsofaflatoxinb1andb2byaqueousextractsoftrachyspermumammi AT iqbalmazhar structuralelucidationandtoxicityassessmentofdegradedproductsofaflatoxinb1andb2byaqueousextractsoftrachyspermumammi AT ghaffarabdul structuralelucidationandtoxicityassessmentofdegradedproductsofaflatoxinb1andb2byaqueousextractsoftrachyspermumammi AT abbasmateen structuralelucidationandtoxicityassessmentofdegradedproductsofaflatoxinb1andb2byaqueousextractsoftrachyspermumammi |