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Construction of recombinant pEGFP-N1-hPer2 plasmid and its expression in osteosarcoma cells
The aim of this study was to construct the eukaryotic expression vector pEGFP-N1-hPer2 and assess its expression in the human osteosarcoma cell line MG63. Total mRNA was extracted from human osteosarcoma MG63 cells, the human period 2 (hPer2) gene was obtained by reverse transcription-polymerase cha...
| Autores principales: | , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
D.A. Spandidos
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4812405/ https://www.ncbi.nlm.nih.gov/pubmed/27073550 http://dx.doi.org/10.3892/ol.2016.4291 |