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Microbial sphingomyelinase induces RhoA-mediated reorganization of the apical brush border membrane and is protective against invasion
The apical brush border membrane (BBM) of intestinal epithelial cells forms a highly structured and dynamic environmental interface that serves to regulate cellular physiology and block invasion by intestinal microbes and their products. How the BBM dynamically responds to pathogenic and commensal b...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4814219/ https://www.ncbi.nlm.nih.gov/pubmed/26864627 http://dx.doi.org/10.1091/mbc.E15-05-0293 |
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author | Saslowsky, David E. Thiagarajah, Jay R. McCormick, Beth A. Lee, Jean C. Lencer, Wayne I. |
author_facet | Saslowsky, David E. Thiagarajah, Jay R. McCormick, Beth A. Lee, Jean C. Lencer, Wayne I. |
author_sort | Saslowsky, David E. |
collection | PubMed |
description | The apical brush border membrane (BBM) of intestinal epithelial cells forms a highly structured and dynamic environmental interface that serves to regulate cellular physiology and block invasion by intestinal microbes and their products. How the BBM dynamically responds to pathogenic and commensal bacterial signals can define intestinal homeostasis and immune function. We previously found that in model intestinal epithelium, the conversion of apical membrane sphingomyelin to ceramide by exogenous bacterial sphingomyelinase (SMase) protected against the endocytosis and toxicity of cholera toxin. Here we elucidate a mechanism of action by showing that SMase induces a dramatic, reversible, RhoA-dependent alteration of the apical cortical F-actin network. Accumulation of apical membrane ceramide is necessary and sufficient to induce the actin phenotype, and this coincides with altered membrane structure and augmented innate immune function as evidenced by resistance to invasion by Salmonella. |
format | Online Article Text |
id | pubmed-4814219 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-48142192016-06-16 Microbial sphingomyelinase induces RhoA-mediated reorganization of the apical brush border membrane and is protective against invasion Saslowsky, David E. Thiagarajah, Jay R. McCormick, Beth A. Lee, Jean C. Lencer, Wayne I. Mol Biol Cell Articles The apical brush border membrane (BBM) of intestinal epithelial cells forms a highly structured and dynamic environmental interface that serves to regulate cellular physiology and block invasion by intestinal microbes and their products. How the BBM dynamically responds to pathogenic and commensal bacterial signals can define intestinal homeostasis and immune function. We previously found that in model intestinal epithelium, the conversion of apical membrane sphingomyelin to ceramide by exogenous bacterial sphingomyelinase (SMase) protected against the endocytosis and toxicity of cholera toxin. Here we elucidate a mechanism of action by showing that SMase induces a dramatic, reversible, RhoA-dependent alteration of the apical cortical F-actin network. Accumulation of apical membrane ceramide is necessary and sufficient to induce the actin phenotype, and this coincides with altered membrane structure and augmented innate immune function as evidenced by resistance to invasion by Salmonella. The American Society for Cell Biology 2016-04-01 /pmc/articles/PMC4814219/ /pubmed/26864627 http://dx.doi.org/10.1091/mbc.E15-05-0293 Text en © 2016 Saslowsky, Thiagarajah, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. |
spellingShingle | Articles Saslowsky, David E. Thiagarajah, Jay R. McCormick, Beth A. Lee, Jean C. Lencer, Wayne I. Microbial sphingomyelinase induces RhoA-mediated reorganization of the apical brush border membrane and is protective against invasion |
title | Microbial sphingomyelinase induces RhoA-mediated reorganization of the apical brush border membrane and is protective against invasion |
title_full | Microbial sphingomyelinase induces RhoA-mediated reorganization of the apical brush border membrane and is protective against invasion |
title_fullStr | Microbial sphingomyelinase induces RhoA-mediated reorganization of the apical brush border membrane and is protective against invasion |
title_full_unstemmed | Microbial sphingomyelinase induces RhoA-mediated reorganization of the apical brush border membrane and is protective against invasion |
title_short | Microbial sphingomyelinase induces RhoA-mediated reorganization of the apical brush border membrane and is protective against invasion |
title_sort | microbial sphingomyelinase induces rhoa-mediated reorganization of the apical brush border membrane and is protective against invasion |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4814219/ https://www.ncbi.nlm.nih.gov/pubmed/26864627 http://dx.doi.org/10.1091/mbc.E15-05-0293 |
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