Bioengineering of Tobacco Mosaic Virus to Create a Non-Infectious Positive Control for Ebola Diagnostic Assays

The 2014 Ebola epidemic is the largest to date. There is no cure or treatment for this deadly disease; therefore there is an urgent need to develop new diagnostics to accurately detect Ebola. Current RT-PCR assays lack sensitive and reliable positive controls. To address this critical need, we devis...

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Autores principales: Lam, Patricia, Gulati, Neetu M., Stewart, Phoebe L., Keri, Ruth A., Steinmetz, Nicole F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4814824/
https://www.ncbi.nlm.nih.gov/pubmed/27030058
http://dx.doi.org/10.1038/srep23803
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author Lam, Patricia
Gulati, Neetu M.
Stewart, Phoebe L.
Keri, Ruth A.
Steinmetz, Nicole F.
author_facet Lam, Patricia
Gulati, Neetu M.
Stewart, Phoebe L.
Keri, Ruth A.
Steinmetz, Nicole F.
author_sort Lam, Patricia
collection PubMed
description The 2014 Ebola epidemic is the largest to date. There is no cure or treatment for this deadly disease; therefore there is an urgent need to develop new diagnostics to accurately detect Ebola. Current RT-PCR assays lack sensitive and reliable positive controls. To address this critical need, we devised a bio-inspired positive control for use in RT-PCR diagnostics: we encapsulated scrambled Ebola RNA sequences inside of tobacco mosaic virus to create a biomimicry that is non-infectious, but stable, and could therefore serve as a positive control in Ebola diagnostic assays. Here, we report the bioengineering and validation of this probe.
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spelling pubmed-48148242016-04-04 Bioengineering of Tobacco Mosaic Virus to Create a Non-Infectious Positive Control for Ebola Diagnostic Assays Lam, Patricia Gulati, Neetu M. Stewart, Phoebe L. Keri, Ruth A. Steinmetz, Nicole F. Sci Rep Article The 2014 Ebola epidemic is the largest to date. There is no cure or treatment for this deadly disease; therefore there is an urgent need to develop new diagnostics to accurately detect Ebola. Current RT-PCR assays lack sensitive and reliable positive controls. To address this critical need, we devised a bio-inspired positive control for use in RT-PCR diagnostics: we encapsulated scrambled Ebola RNA sequences inside of tobacco mosaic virus to create a biomimicry that is non-infectious, but stable, and could therefore serve as a positive control in Ebola diagnostic assays. Here, we report the bioengineering and validation of this probe. Nature Publishing Group 2016-03-31 /pmc/articles/PMC4814824/ /pubmed/27030058 http://dx.doi.org/10.1038/srep23803 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Lam, Patricia
Gulati, Neetu M.
Stewart, Phoebe L.
Keri, Ruth A.
Steinmetz, Nicole F.
Bioengineering of Tobacco Mosaic Virus to Create a Non-Infectious Positive Control for Ebola Diagnostic Assays
title Bioengineering of Tobacco Mosaic Virus to Create a Non-Infectious Positive Control for Ebola Diagnostic Assays
title_full Bioengineering of Tobacco Mosaic Virus to Create a Non-Infectious Positive Control for Ebola Diagnostic Assays
title_fullStr Bioengineering of Tobacco Mosaic Virus to Create a Non-Infectious Positive Control for Ebola Diagnostic Assays
title_full_unstemmed Bioengineering of Tobacco Mosaic Virus to Create a Non-Infectious Positive Control for Ebola Diagnostic Assays
title_short Bioengineering of Tobacco Mosaic Virus to Create a Non-Infectious Positive Control for Ebola Diagnostic Assays
title_sort bioengineering of tobacco mosaic virus to create a non-infectious positive control for ebola diagnostic assays
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4814824/
https://www.ncbi.nlm.nih.gov/pubmed/27030058
http://dx.doi.org/10.1038/srep23803
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