Enhanced thermal stability of lichenase from Bacillus subtilis 168 by SpyTag/SpyCatcher-mediated spontaneous cyclization

BACKGROUND: SpyTag is a peptide that can form an irreversible covalent linkage to its 12 kDa partner SpyCatcher via a spontaneous isopeptide bond. Herein, we fused SpyTag at the N-terminal of lichenase and SpyCatcher at C-terminal so that the termini of lichenase were locked together by the covalent...

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Detalles Bibliográficos
Autores principales: Wang, Jindan, Wang, Yilin, Wang, Xinzhe, Zhang, Dandan, Wu, Shuyu, Zhang, Guangya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4815112/
https://www.ncbi.nlm.nih.gov/pubmed/27034717
http://dx.doi.org/10.1186/s13068-016-0490-5

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4815112/
https://www.ncbi.nlm.nih.gov/pubmed/27034717
http://dx.doi.org/10.1186/s13068-016-0490-5

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