Nucleic acids delivery methods for genome editing in zygotes and embryos: the old, the new, and the old-new

In the recent years, sequence-specific nucleases such as ZFNs, TALENs, and CRISPR/Cas9 have revolutionzed the fields of animal genome editing and transgenesis. However, these new techniques require microinjection to deliver nucleic acids into embryos to generate gene-modified animals. Microinjection...

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Detalles Bibliográficos
Autores principales: Sato, Masahiro, Ohtsuka, Masato, Watanabe, Satoshi, Gurumurthy, Channabasavaiah B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4815204/
https://www.ncbi.nlm.nih.gov/pubmed/27037013
http://dx.doi.org/10.1186/s13062-016-0115-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4815204/
https://www.ncbi.nlm.nih.gov/pubmed/27037013
http://dx.doi.org/10.1186/s13062-016-0115-8

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