Cargando…

Enhanced Methylation Analysis by Recovery of Unsequenceable Fragments

Bisulfite sequencing is a valuable tool for mapping the position of 5-methylcytosine in the genome at single base resolution. However, the associated chemical treatment causes strand scission, which depletes the number of sequenceable DNA fragments in a library and thus necessitates PCR amplificatio...

Descripción completa

Detalles Bibliográficos
Autores principales:	McInroy, Gordon R., Beraldi, Dario, Raiber, Eun-Ang, Modrzynska, Katarzyna, van Delft, Pieter, Billker, Oliver, Balasubramanian, Shankar
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2016
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4816320/ https://www.ncbi.nlm.nih.gov/pubmed/27031619 http://dx.doi.org/10.1371/journal.pone.0152322

Ejemplares similares

Chemical biology of genomic DNA: minimizing PCR bias†
por: McInroy, Gordon R., et al.
Publicado: (2014)

Genome-wide mapping of 5-hydroxymethyluracil in the eukaryote parasite Leishmania
por: Kawasaki, Fumiko, et al.
Publicado: (2017)

In vivo genome-wide profiling reveals a tissue-specific role for 5-formylcytosine
por: Iurlaro, Mario, et al.
Publicado: (2016)

An Epigenetics‐Inspired DNA‐Based Data Storage System
por: Mayer, Clemens, et al.
Publicado: (2016)

Base resolution maps reveal the importance of 5-hydroxymethylcytosine in a human glioblastoma
por: Raiber, Eun-Ang, et al.
Publicado: (2017)

5-Formylcytosine alters the structure of the DNA double helix
por: Raiber, Eun-Ang, et al.
Publicado: (2014)

Chemical Methods for Decoding Cytosine Modifications in DNA
por: Booth, Michael J., et al.
Publicado: (2014)

Genome-wide distribution of 5-formylcytosine in embryonic stem cells is associated with transcription and depends on thymine DNA glycosylase
por: Raiber, Eun-Ang, et al.
Publicado: (2012)

Sequencing the unsequenceable: Expanded CGG-repeat alleles of the fragile X gene
por: Loomis, Erick W., et al.
Publicado: (2013)

Interrogating the “unsequenceable” genomic trinucleotide repeat disorders by long-read sequencing
por: Liu, Qian, et al.
Publicado: (2017)

A non-canonical DNA structure is a binding motif for the transcription factor SP1 in vitro
por: Raiber, Eun-Ang, et al.
Publicado: (2012)

Investigating the Acid Site Distribution of a New-Generation Methyl Chloride Synthesis Catalyst
por: McInroy, Alastair R., et al.
Publicado: (2019)

Proteomic Profiling and Protein Identification by MALDI-TOF Mass Spectrometry in Unsequenced Parasitic Nematodes
por: Millares, Paul, et al.
Publicado: (2012)

Inducible developmental reprogramming redefines commitment to sexual development in the malaria parasite Plasmodium berghei
por: Kent, Robyn S, et al.
Publicado: (2018)

Selective RNAVersus DNA G-Quadruplex Targeting by In Situ Click Chemistry**
por: Di Antonio, Marco, et al.
Publicado: (2012)

Examination of Triacylglycerol Biosynthetic Pathways via De Novo Transcriptomic and Proteomic Analyses in an Unsequenced Microalga
por: Guarnieri, Michael T., et al.
Publicado: (2011)

A constrained SSU-rRNA phylogeny reveals the unsequenced diversity of photosynthetic Cyanobacteria (Oxyphotobacteria)
por: Cornet, Luc, et al.
Publicado: (2018)

The Utility of Genomic and Transcriptomic Data in the Construction of Proxy Protein Sequence Databases for Unsequenced Tree Nuts
por: Pirone-Davies, Cary, et al.
Publicado: (2020)

2′-O-Methyl-5-hydroxymethylcytidine: A Second Oxidative Derivative of 5-Methylcytidine in RNA
por: Huber, Sabrina M., et al.
Publicado: (2017)

Comparative RNA-seq Analysis in the Unsequenced Axolotl: The Oncogene Burst Highlights Early Gene Expression in the Blastema
por: Stewart, Ron, et al.
Publicado: (2013)

Describing a Clinical Group Coding Method for Identifying Competencies in an Allied Health Single Session
por: Craig, Shelley L, et al.
Publicado: (2020)

Homology-Driven Proteomics of Dinoflagellates with Unsequenced Genomes Using MALDI-TOF/TOF and Automated De Novo Sequencing
por: Wang, Da-Zhi, et al.
Publicado: (2011)

Dichloromethane Degradation Pathway from Unsequenced Hyphomicrobium sp. MC8b Rapidly Explored by Pan-Proteomics
por: Hayoun, Karim, et al.
Publicado: (2020)

G-Quadruplex structures are stable and detectable in human genomic DNA
por: Lam, Enid Yi Ni, et al.
Publicado: (2013)

Differential Requirement for Cathepsin D for Processing of the Full Length and C-Terminal Fragment of the Malaria Antigen MSP1
por: Tulone, Calogero, et al.
Publicado: (2011)

A Stem Cell Strategy Identifies Glycophorin C as a Major Erythrocyte Receptor for the Rodent Malaria Parasite Plasmodium berghei
por: Yiangou, Loukia, et al.
Publicado: (2016)

Genomic characterization of 99 viruses from the bunyavirus families Nairoviridae, Peribunyaviridae, and Phenuiviridae, including 35 previously unsequenced viruses
por: Kapuscinski, Marylee L., et al.
Publicado: (2021)

Symptoms of Fern Distortion Syndrome Resulting from Inoculation with Opportunistic Endophytic Fluorescent Pseudomonas spp.
por: Kloepper, Joseph W., et al.
Publicado: (2013)

A screen for hydroxymethylcytosine and formylcytosine binding proteins suggests functions in transcription and chromatin regulation
por: Iurlaro, Mario, et al.
Publicado: (2013)

Beyond a shared experience: Queer and trans youth navigating COVID-19
por: Paceley, Megan S, et al.
Publicado: (2021)

Randomized experiment on the effect of incentives and mailing strategy on response rates in a mail survey of dentists
por: McKernan, Susan C., et al.
Publicado: (2022)

Genome-wide mapping of FOXM1 binding reveals co-binding with estrogen receptor alpha in breast cancer cells
por: Sanders, Deborah A, et al.
Publicado: (2013)

Pectin-Rich Amendment Enhances Soybean Growth Promotion and Nodulation Mediated by Bacillus Velezensis Strains
por: Hassan, Mohammad K., et al.
Publicado: (2019)

Enhancing genetic mapping of complex genomes through the design of highly-multiplexed SNP arrays: application to the large and unsequenced genomes of white spruce and black spruce
por: Pavy, Nathalie, et al.
Publicado: (2008)

Biological control of Heterodera glycines by spore-forming plant growth-promoting rhizobacteria (PGPR) on soybean
por: Xiang, Ni, et al.
Publicado: (2017)

Demonstrating LGBTQ+ Affirmative Practice in Groups:: Developing Competence through Simulation-Based Learning
por: Craig, Shelley L., et al.
Publicado: (2022)

Interaction of Methanol over CsCl- and KCl-Doped η-Alumina and the Attenuation of Dimethyl Ether Formation
por: McInroy, Alastair R., et al.
Publicado: (2022)

Large scale single nucleotide polymorphism discovery in unsequenced genomes using second generation high throughput sequencing technology: applied to turkey
por: Kerstens, Hindrik HD, et al.
Publicado: (2009)

Variation in Bat Guano Bacterial Community Composition With Depth
por: Newman, Molli M., et al.
Publicado: (2018)

How has COVID‐19 impacted on Learning Disability Nursing Services in Scotland?
por: Middleton, Alan, et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_c8j9hl5pln273ke11u3pu1psgt