Cas9 Functionally Opens Chromatin

Using a nuclease-dead Cas9 mutant, we show that Cas9 reproducibly induces chromatin accessibility at previously inaccessible genomic loci. Cas9 chromatin opening is sufficient to enable adjacent binding and transcriptional activation by the settler transcription factor retinoic acid receptor at prev...

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Detalles Bibliográficos
Autores principales: Barkal, Amira A., Srinivasan, Sharanya, Hashimoto, Tatsunori, Gifford, David K., Sherwood, Richard I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4816323/
https://www.ncbi.nlm.nih.gov/pubmed/27031353
http://dx.doi.org/10.1371/journal.pone.0152683

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4816323/
https://www.ncbi.nlm.nih.gov/pubmed/27031353
http://dx.doi.org/10.1371/journal.pone.0152683

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