Cas9 Functionally Opens Chromatin

Using a nuclease-dead Cas9 mutant, we show that Cas9 reproducibly induces chromatin accessibility at previously inaccessible genomic loci. Cas9 chromatin opening is sufficient to enable adjacent binding and transcriptional activation by the settler transcription factor retinoic acid receptor at prev...

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Detalles Bibliográficos
Autores principales: Barkal, Amira A., Srinivasan, Sharanya, Hashimoto, Tatsunori, Gifford, David K., Sherwood, Richard I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4816323/
https://www.ncbi.nlm.nih.gov/pubmed/27031353
http://dx.doi.org/10.1371/journal.pone.0152683
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author Barkal, Amira A.
Srinivasan, Sharanya
Hashimoto, Tatsunori
Gifford, David K.
Sherwood, Richard I.
author_facet Barkal, Amira A.
Srinivasan, Sharanya
Hashimoto, Tatsunori
Gifford, David K.
Sherwood, Richard I.
author_sort Barkal, Amira A.
collection PubMed
description Using a nuclease-dead Cas9 mutant, we show that Cas9 reproducibly induces chromatin accessibility at previously inaccessible genomic loci. Cas9 chromatin opening is sufficient to enable adjacent binding and transcriptional activation by the settler transcription factor retinoic acid receptor at previously unbound motifs. Thus, we demonstrate a new use for Cas9 in increasing surrounding chromatin accessibility to alter local transcription factor binding.
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spelling pubmed-48163232016-04-14 Cas9 Functionally Opens Chromatin Barkal, Amira A. Srinivasan, Sharanya Hashimoto, Tatsunori Gifford, David K. Sherwood, Richard I. PLoS One Research Article Using a nuclease-dead Cas9 mutant, we show that Cas9 reproducibly induces chromatin accessibility at previously inaccessible genomic loci. Cas9 chromatin opening is sufficient to enable adjacent binding and transcriptional activation by the settler transcription factor retinoic acid receptor at previously unbound motifs. Thus, we demonstrate a new use for Cas9 in increasing surrounding chromatin accessibility to alter local transcription factor binding. Public Library of Science 2016-03-31 /pmc/articles/PMC4816323/ /pubmed/27031353 http://dx.doi.org/10.1371/journal.pone.0152683 Text en © 2016 Barkal et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Barkal, Amira A.
Srinivasan, Sharanya
Hashimoto, Tatsunori
Gifford, David K.
Sherwood, Richard I.
Cas9 Functionally Opens Chromatin
title Cas9 Functionally Opens Chromatin
title_full Cas9 Functionally Opens Chromatin
title_fullStr Cas9 Functionally Opens Chromatin
title_full_unstemmed Cas9 Functionally Opens Chromatin
title_short Cas9 Functionally Opens Chromatin
title_sort cas9 functionally opens chromatin
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4816323/
https://www.ncbi.nlm.nih.gov/pubmed/27031353
http://dx.doi.org/10.1371/journal.pone.0152683
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