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Diagonally Scanned Light-Sheet Microscopy for Fast Volumetric Imaging of Adherent Cells

In subcellular light-sheet fluorescence microscopy (LSFM) of adherent cells, glass substrates are advantageously rotated relative to the excitation and emission light paths to avoid glass-induced optical aberrations. Because cells are spread across the sample volume, three-dimensional imaging requir...

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Detalles Bibliográficos
Autores principales: Dean, Kevin M., Roudot, Philippe, Reis, Carlos R., Welf, Erik S., Mettlen, Marcel, Fiolka, Reto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Biophysical Society 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4816690/
https://www.ncbi.nlm.nih.gov/pubmed/27028654
http://dx.doi.org/10.1016/j.bpj.2016.01.029