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Diagonally Scanned Light-Sheet Microscopy for Fast Volumetric Imaging of Adherent Cells
In subcellular light-sheet fluorescence microscopy (LSFM) of adherent cells, glass substrates are advantageously rotated relative to the excitation and emission light paths to avoid glass-induced optical aberrations. Because cells are spread across the sample volume, three-dimensional imaging requir...
Autores principales: | Dean, Kevin M., Roudot, Philippe, Reis, Carlos R., Welf, Erik S., Mettlen, Marcel, Fiolka, Reto |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Biophysical Society
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4816690/ https://www.ncbi.nlm.nih.gov/pubmed/27028654 http://dx.doi.org/10.1016/j.bpj.2016.01.029 |
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