Tracking of Normal and Malignant Progenitor Cell Cycle Transit in a Defined Niche

While implicated in therapeutic resistance, malignant progenitor cell cycle kinetics have been difficult to quantify in real-time. We developed an efficient lentiviral bicistronic fluorescent, ubiquitination-based cell cycle indicator reporter (Fucci2BL) to image live single progenitors on a defined...

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Autores principales: Pineda, Gabriel, Lennon, Kathleen M., Delos Santos, Nathaniel P., Lambert-Fliszar, Florence, Riso, Gennarina L., Lazzari, Elisa, Marra, Marco A., Morris, Sheldon, Sakaue-Sawano, Asako, Miyawaki, Atsushi, Jamieson, Catriona H. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4819192/
https://www.ncbi.nlm.nih.gov/pubmed/27041210
http://dx.doi.org/10.1038/srep23885
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author Pineda, Gabriel
Lennon, Kathleen M.
Delos Santos, Nathaniel P.
Lambert-Fliszar, Florence
Riso, Gennarina L.
Lazzari, Elisa
Marra, Marco A.
Morris, Sheldon
Sakaue-Sawano, Asako
Miyawaki, Atsushi
Jamieson, Catriona H. M.
author_facet Pineda, Gabriel
Lennon, Kathleen M.
Delos Santos, Nathaniel P.
Lambert-Fliszar, Florence
Riso, Gennarina L.
Lazzari, Elisa
Marra, Marco A.
Morris, Sheldon
Sakaue-Sawano, Asako
Miyawaki, Atsushi
Jamieson, Catriona H. M.
author_sort Pineda, Gabriel
collection PubMed
description While implicated in therapeutic resistance, malignant progenitor cell cycle kinetics have been difficult to quantify in real-time. We developed an efficient lentiviral bicistronic fluorescent, ubiquitination-based cell cycle indicator reporter (Fucci2BL) to image live single progenitors on a defined niche coupled with cell cycle gene expression analysis. We have identified key differences in cell cycle regulatory gene expression and transit times between normal and chronic myeloid leukemia progenitors that may inform cancer stem cell eradication strategies.
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spelling pubmed-48191922016-04-06 Tracking of Normal and Malignant Progenitor Cell Cycle Transit in a Defined Niche Pineda, Gabriel Lennon, Kathleen M. Delos Santos, Nathaniel P. Lambert-Fliszar, Florence Riso, Gennarina L. Lazzari, Elisa Marra, Marco A. Morris, Sheldon Sakaue-Sawano, Asako Miyawaki, Atsushi Jamieson, Catriona H. M. Sci Rep Article While implicated in therapeutic resistance, malignant progenitor cell cycle kinetics have been difficult to quantify in real-time. We developed an efficient lentiviral bicistronic fluorescent, ubiquitination-based cell cycle indicator reporter (Fucci2BL) to image live single progenitors on a defined niche coupled with cell cycle gene expression analysis. We have identified key differences in cell cycle regulatory gene expression and transit times between normal and chronic myeloid leukemia progenitors that may inform cancer stem cell eradication strategies. Nature Publishing Group 2016-04-04 /pmc/articles/PMC4819192/ /pubmed/27041210 http://dx.doi.org/10.1038/srep23885 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Pineda, Gabriel
Lennon, Kathleen M.
Delos Santos, Nathaniel P.
Lambert-Fliszar, Florence
Riso, Gennarina L.
Lazzari, Elisa
Marra, Marco A.
Morris, Sheldon
Sakaue-Sawano, Asako
Miyawaki, Atsushi
Jamieson, Catriona H. M.
Tracking of Normal and Malignant Progenitor Cell Cycle Transit in a Defined Niche
title Tracking of Normal and Malignant Progenitor Cell Cycle Transit in a Defined Niche
title_full Tracking of Normal and Malignant Progenitor Cell Cycle Transit in a Defined Niche
title_fullStr Tracking of Normal and Malignant Progenitor Cell Cycle Transit in a Defined Niche
title_full_unstemmed Tracking of Normal and Malignant Progenitor Cell Cycle Transit in a Defined Niche
title_short Tracking of Normal and Malignant Progenitor Cell Cycle Transit in a Defined Niche
title_sort tracking of normal and malignant progenitor cell cycle transit in a defined niche
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4819192/
https://www.ncbi.nlm.nih.gov/pubmed/27041210
http://dx.doi.org/10.1038/srep23885
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