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Mutanalyst, an online tool for assessing the mutational spectrum of epPCR libraries with poor sampling
BACKGROUND: Assessing library diversity is an important control step in a directed evolution experiment. To do this, a limited amount of colonies from a test library are sequenced and tested. In the case of an error-prone PCR library, the spectrum of the identified mutations — the proportions of mut...
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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BioMed Central
2016
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4820924/ https://www.ncbi.nlm.nih.gov/pubmed/27044645 http://dx.doi.org/10.1186/s12859-016-0996-7 |