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A Molecular Chipper technology for CRISPR sgRNA library generation and functional mapping of noncoding regions

Clustered regularly-interspaced palindromic repeats (CRISPR)-based genetic screens using single-guide-RNA (sgRNA) libraries have proven powerful to identify genetic regulators. Applying CRISPR screens to interrogate functional elements in noncoding regions requires generating sgRNA libraries that ar...

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Detalles Bibliográficos
Autores principales:	Cheng, Jijun, Roden, Christine A., Pan, Wen, Zhu, Shu, Baccei, Anna, Pan, Xinghua, Jiang, Tingting, Kluger, Yuval, Weissman, Sherman M., Guo, Shangqin, Flavell, Richard A., Ding, Ye, Lu, Jun
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2016
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4820989/ https://www.ncbi.nlm.nih.gov/pubmed/27025950 http://dx.doi.org/10.1038/ncomms11178

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