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Removal of between-run variation in a multi-plate qPCR experiment

Quantitative PCR (qPCR) is the method of choice in gene expression analysis. However, the number of groups or treatments, target genes and technical replicates quickly exceeds the capacity of a single run on a qPCR machine and the measurements have to be spread over more than 1 plate. Such multi-pla...

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Detalles Bibliográficos
Autores principales: Ruijter, Jan M., Ruiz Villalba, Adrián, Hellemans, Jan, Untergasser, Andreas, van den Hoff, Maurice J.B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4822202/
https://www.ncbi.nlm.nih.gov/pubmed/27077038
http://dx.doi.org/10.1016/j.bdq.2015.07.001