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Removal of between-run variation in a multi-plate qPCR experiment
Quantitative PCR (qPCR) is the method of choice in gene expression analysis. However, the number of groups or treatments, target genes and technical replicates quickly exceeds the capacity of a single run on a qPCR machine and the measurements have to be spread over more than 1 plate. Such multi-pla...
Autores principales: | Ruijter, Jan M., Ruiz Villalba, Adrián, Hellemans, Jan, Untergasser, Andreas, van den Hoff, Maurice J.B. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4822202/ https://www.ncbi.nlm.nih.gov/pubmed/27077038 http://dx.doi.org/10.1016/j.bdq.2015.07.001 |
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