How good is a PCR efficiency estimate: Recommendations for precise and robust qPCR efficiency assessments

We have examined the imprecision in the estimation of PCR efficiency by means of standard curves based on strategic experimental design with large number of technical replicates. In particular, how robust this estimation is in terms of a commonly varying factors: the instrument used, the number of t...

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Detalles Bibliográficos
Autores principales: Svec, David, Tichopad, Ales, Novosadova, Vendula, Pfaffl, Michael W., Kubista, Mikael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4822216/
https://www.ncbi.nlm.nih.gov/pubmed/27077029
http://dx.doi.org/10.1016/j.bdq.2015.01.005

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4822216/
https://www.ncbi.nlm.nih.gov/pubmed/27077029
http://dx.doi.org/10.1016/j.bdq.2015.01.005

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