Cargando…

Antioxidant and Anti-Inflammatory Activities of Unexplored Brazilian Native Fruits

Brazilian native fruits are unmatched in their variety, but a poorly explored resource for the development of food and pharmaceutical products. The aim of this study was to evaluate the phenolic composition as well as the antioxidant and anti-inflammatory activities of the extracts of leaves, seeds,...

Descripción completa

Detalles Bibliográficos
Autores principales: Infante, Juliana, Rosalen, Pedro Luiz, Lazarini, Josy Goldoni, Franchin, Marcelo, de Alencar, Severino Matias
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4822956/
https://www.ncbi.nlm.nih.gov/pubmed/27050817
http://dx.doi.org/10.1371/journal.pone.0152974
_version_ 1782425834369318912
author Infante, Juliana
Rosalen, Pedro Luiz
Lazarini, Josy Goldoni
Franchin, Marcelo
de Alencar, Severino Matias
author_facet Infante, Juliana
Rosalen, Pedro Luiz
Lazarini, Josy Goldoni
Franchin, Marcelo
de Alencar, Severino Matias
author_sort Infante, Juliana
collection PubMed
description Brazilian native fruits are unmatched in their variety, but a poorly explored resource for the development of food and pharmaceutical products. The aim of this study was to evaluate the phenolic composition as well as the antioxidant and anti-inflammatory activities of the extracts of leaves, seeds, and pulp of four Brazilian native fruits (Eugenia leitonii, Eugenia involucrata, Eugenia brasiliensis, and Eugenia myrcianthes). GC—MS analyses of the ethanolic extracts showed the presence of epicatechin and gallic acid as the major compounds in these fruits. Antioxidant activity was measured using synthetic DPPH free-radical scavenging, β-carotene bleaching assay, and reactive oxygen species (ROO·, O(2)·(−), and HOCl). The fruit extracts also exhibited antioxidant effect against biologically relevant radicals such as peroxyl, superoxide, and hypochlorous acid. In general, the pulps were the fruit fractions that exhibited the lowest antioxidant activities, whereas the leaves showed the highest ones. The anti-inflammatory activity was assessed in an in vivo model using the carrageenan-induced neutrophil migration assay, which evaluates the inflammatory response in the acute phase. The pulp, seeds, and leaves of these fruits reduced the neutrophil influx by 40% to 64%. Based on these results, we suggest that the anti-inflammatory activity of these native fruits is related to the modulation of neutrophil migration, through the inhibition of cytokines, chemokines, and adhesion molecules, as well as to the antioxidant action of their ethanolic extracts in scavenging the free-radicals released by neutrophils. Therefore, these native fruits can be useful to produce food additives and functional foods.
format Online
Article
Text
id pubmed-4822956
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-48229562016-04-22 Antioxidant and Anti-Inflammatory Activities of Unexplored Brazilian Native Fruits Infante, Juliana Rosalen, Pedro Luiz Lazarini, Josy Goldoni Franchin, Marcelo de Alencar, Severino Matias PLoS One Research Article Brazilian native fruits are unmatched in their variety, but a poorly explored resource for the development of food and pharmaceutical products. The aim of this study was to evaluate the phenolic composition as well as the antioxidant and anti-inflammatory activities of the extracts of leaves, seeds, and pulp of four Brazilian native fruits (Eugenia leitonii, Eugenia involucrata, Eugenia brasiliensis, and Eugenia myrcianthes). GC—MS analyses of the ethanolic extracts showed the presence of epicatechin and gallic acid as the major compounds in these fruits. Antioxidant activity was measured using synthetic DPPH free-radical scavenging, β-carotene bleaching assay, and reactive oxygen species (ROO·, O(2)·(−), and HOCl). The fruit extracts also exhibited antioxidant effect against biologically relevant radicals such as peroxyl, superoxide, and hypochlorous acid. In general, the pulps were the fruit fractions that exhibited the lowest antioxidant activities, whereas the leaves showed the highest ones. The anti-inflammatory activity was assessed in an in vivo model using the carrageenan-induced neutrophil migration assay, which evaluates the inflammatory response in the acute phase. The pulp, seeds, and leaves of these fruits reduced the neutrophil influx by 40% to 64%. Based on these results, we suggest that the anti-inflammatory activity of these native fruits is related to the modulation of neutrophil migration, through the inhibition of cytokines, chemokines, and adhesion molecules, as well as to the antioxidant action of their ethanolic extracts in scavenging the free-radicals released by neutrophils. Therefore, these native fruits can be useful to produce food additives and functional foods. Public Library of Science 2016-04-06 /pmc/articles/PMC4822956/ /pubmed/27050817 http://dx.doi.org/10.1371/journal.pone.0152974 Text en © 2016 Infante et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Infante, Juliana
Rosalen, Pedro Luiz
Lazarini, Josy Goldoni
Franchin, Marcelo
de Alencar, Severino Matias
Antioxidant and Anti-Inflammatory Activities of Unexplored Brazilian Native Fruits
title Antioxidant and Anti-Inflammatory Activities of Unexplored Brazilian Native Fruits
title_full Antioxidant and Anti-Inflammatory Activities of Unexplored Brazilian Native Fruits
title_fullStr Antioxidant and Anti-Inflammatory Activities of Unexplored Brazilian Native Fruits
title_full_unstemmed Antioxidant and Anti-Inflammatory Activities of Unexplored Brazilian Native Fruits
title_short Antioxidant and Anti-Inflammatory Activities of Unexplored Brazilian Native Fruits
title_sort antioxidant and anti-inflammatory activities of unexplored brazilian native fruits
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4822956/
https://www.ncbi.nlm.nih.gov/pubmed/27050817
http://dx.doi.org/10.1371/journal.pone.0152974
work_keys_str_mv AT infantejuliana antioxidantandantiinflammatoryactivitiesofunexploredbraziliannativefruits
AT rosalenpedroluiz antioxidantandantiinflammatoryactivitiesofunexploredbraziliannativefruits
AT lazarinijosygoldoni antioxidantandantiinflammatoryactivitiesofunexploredbraziliannativefruits
AT franchinmarcelo antioxidantandantiinflammatoryactivitiesofunexploredbraziliannativefruits
AT dealencarseverinomatias antioxidantandantiinflammatoryactivitiesofunexploredbraziliannativefruits