Potential use of bacterial community succession for estimating post-mortem interval as revealed by high-throughput sequencing

Decomposition is a complex process involving the interaction of both biotic and abiotic factors. Microbes play a critical role in the process of carrion decomposition. In this study, we analysed bacterial communities from live rats and rat remains decomposed under natural conditions, or excluding sa...

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Autores principales: Guo, Juanjuan, Fu, Xiaoliang, Liao, Huidan, Hu, Zhenyu, Long, Lingling, Yan, Weitao, Ding, Yanjun, Zha, Lagabaiyila, Guo, Yadong, Yan, Jie, Chang, Yunfeng, Cai, Jifeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4823735/
https://www.ncbi.nlm.nih.gov/pubmed/27052375
http://dx.doi.org/10.1038/srep24197
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author Guo, Juanjuan
Fu, Xiaoliang
Liao, Huidan
Hu, Zhenyu
Long, Lingling
Yan, Weitao
Ding, Yanjun
Zha, Lagabaiyila
Guo, Yadong
Yan, Jie
Chang, Yunfeng
Cai, Jifeng
author_facet Guo, Juanjuan
Fu, Xiaoliang
Liao, Huidan
Hu, Zhenyu
Long, Lingling
Yan, Weitao
Ding, Yanjun
Zha, Lagabaiyila
Guo, Yadong
Yan, Jie
Chang, Yunfeng
Cai, Jifeng
author_sort Guo, Juanjuan
collection PubMed
description Decomposition is a complex process involving the interaction of both biotic and abiotic factors. Microbes play a critical role in the process of carrion decomposition. In this study, we analysed bacterial communities from live rats and rat remains decomposed under natural conditions, or excluding sarcosaphagous insect interference, in China using Illumina MiSeq sequencing of 16S rRNA gene amplicons. A total of 1,394,842 high-quality sequences and 1,938 singleton operational taxonomic units were obtained. Bacterial communities showed notable variation in relative abundance and became more similar to each other across body sites during the decomposition process. As decomposition progressed, Proteobacteria (mostly Gammaproteobacteria) became the predominant phylum in both the buccal cavity and rectum, while Firmicutes and Bacteroidetes in the mouth and rectum, respectively, gradually decreased. In particular, the arrival and oviposition of sarcosaphagous insects had no obvious influence on bacterial taxa composition, but accelerated the loss of biomass. In contrast to the rectum, the microbial community structure in the buccal cavity of live rats differed considerably from that of rats immediately after death. Although this research indicates that bacterial communities can be used as a “microbial clock” for the estimation of post-mortem interval, further work is required to better understand this concept.
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spelling pubmed-48237352016-04-18 Potential use of bacterial community succession for estimating post-mortem interval as revealed by high-throughput sequencing Guo, Juanjuan Fu, Xiaoliang Liao, Huidan Hu, Zhenyu Long, Lingling Yan, Weitao Ding, Yanjun Zha, Lagabaiyila Guo, Yadong Yan, Jie Chang, Yunfeng Cai, Jifeng Sci Rep Article Decomposition is a complex process involving the interaction of both biotic and abiotic factors. Microbes play a critical role in the process of carrion decomposition. In this study, we analysed bacterial communities from live rats and rat remains decomposed under natural conditions, or excluding sarcosaphagous insect interference, in China using Illumina MiSeq sequencing of 16S rRNA gene amplicons. A total of 1,394,842 high-quality sequences and 1,938 singleton operational taxonomic units were obtained. Bacterial communities showed notable variation in relative abundance and became more similar to each other across body sites during the decomposition process. As decomposition progressed, Proteobacteria (mostly Gammaproteobacteria) became the predominant phylum in both the buccal cavity and rectum, while Firmicutes and Bacteroidetes in the mouth and rectum, respectively, gradually decreased. In particular, the arrival and oviposition of sarcosaphagous insects had no obvious influence on bacterial taxa composition, but accelerated the loss of biomass. In contrast to the rectum, the microbial community structure in the buccal cavity of live rats differed considerably from that of rats immediately after death. Although this research indicates that bacterial communities can be used as a “microbial clock” for the estimation of post-mortem interval, further work is required to better understand this concept. Nature Publishing Group 2016-04-07 /pmc/articles/PMC4823735/ /pubmed/27052375 http://dx.doi.org/10.1038/srep24197 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Guo, Juanjuan
Fu, Xiaoliang
Liao, Huidan
Hu, Zhenyu
Long, Lingling
Yan, Weitao
Ding, Yanjun
Zha, Lagabaiyila
Guo, Yadong
Yan, Jie
Chang, Yunfeng
Cai, Jifeng
Potential use of bacterial community succession for estimating post-mortem interval as revealed by high-throughput sequencing
title Potential use of bacterial community succession for estimating post-mortem interval as revealed by high-throughput sequencing
title_full Potential use of bacterial community succession for estimating post-mortem interval as revealed by high-throughput sequencing
title_fullStr Potential use of bacterial community succession for estimating post-mortem interval as revealed by high-throughput sequencing
title_full_unstemmed Potential use of bacterial community succession for estimating post-mortem interval as revealed by high-throughput sequencing
title_short Potential use of bacterial community succession for estimating post-mortem interval as revealed by high-throughput sequencing
title_sort potential use of bacterial community succession for estimating post-mortem interval as revealed by high-throughput sequencing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4823735/
https://www.ncbi.nlm.nih.gov/pubmed/27052375
http://dx.doi.org/10.1038/srep24197
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