Generation of KCL013 research grade human embryonic stem cell line carrying a mutation in the HTT gene

The KCL013 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation affecting one allele of the HTT gene encoding huntingtin (42 trinucleotide repeats; 17 for the normal allele). The ICM was isolated using laser microsurgery and plate...

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Autores principales: Jacquet, Laureen, Hewitson, Heema, Wood, Victoria, Kadeva, Neli, Cornwell, Glenda, Codognotto, Stefano, Stephenson, Emma, Ilic, Dusko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Lab Resource: Stem Cell Line
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4823765/
https://www.ncbi.nlm.nih.gov/pubmed/27345987
http://dx.doi.org/10.1016/j.scr.2016.01.011
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author Jacquet, Laureen
Hewitson, Heema
Wood, Victoria
Kadeva, Neli
Cornwell, Glenda
Codognotto, Stefano
Stephenson, Emma
Ilic, Dusko
author_facet Jacquet, Laureen
Hewitson, Heema
Wood, Victoria
Kadeva, Neli
Cornwell, Glenda
Codognotto, Stefano
Stephenson, Emma
Ilic, Dusko
author_sort Jacquet, Laureen
collection PubMed
description The KCL013 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation affecting one allele of the HTT gene encoding huntingtin (42 trinucleotide repeats; 17 for the normal allele). The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro assays.
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spelling pubmed-48237652016-04-15 Generation of KCL013 research grade human embryonic stem cell line carrying a mutation in the HTT gene Jacquet, Laureen Hewitson, Heema Wood, Victoria Kadeva, Neli Cornwell, Glenda Codognotto, Stefano Stephenson, Emma Ilic, Dusko Stem Cell Res Lab Resource: Stem Cell Line The KCL013 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation affecting one allele of the HTT gene encoding huntingtin (42 trinucleotide repeats; 17 for the normal allele). The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro assays. Elsevier 2016-03 /pmc/articles/PMC4823765/ /pubmed/27345987 http://dx.doi.org/10.1016/j.scr.2016.01.011 Text en © 2016 University of Texas at Austin Dell Medical School http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Lab Resource: Stem Cell Line
Jacquet, Laureen
Hewitson, Heema
Wood, Victoria
Kadeva, Neli
Cornwell, Glenda
Codognotto, Stefano
Stephenson, Emma
Ilic, Dusko
Generation of KCL013 research grade human embryonic stem cell line carrying a mutation in the HTT gene
title Generation of KCL013 research grade human embryonic stem cell line carrying a mutation in the HTT gene
title_full Generation of KCL013 research grade human embryonic stem cell line carrying a mutation in the HTT gene
title_fullStr Generation of KCL013 research grade human embryonic stem cell line carrying a mutation in the HTT gene
title_full_unstemmed Generation of KCL013 research grade human embryonic stem cell line carrying a mutation in the HTT gene
title_short Generation of KCL013 research grade human embryonic stem cell line carrying a mutation in the HTT gene
title_sort generation of kcl013 research grade human embryonic stem cell line carrying a mutation in the htt gene
topic Lab Resource: Stem Cell Line
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4823765/
https://www.ncbi.nlm.nih.gov/pubmed/27345987
http://dx.doi.org/10.1016/j.scr.2016.01.011
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