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Where does transcription start? 5′-RACE adapted to next-generation sequencing
The variability and complexity of the transcription initiation process was examined by adapting RNA ligase-mediated rapid amplification of 5′ cDNA ends (5′-RACE) to Next-Generation Sequencing (NGS). We oligo-labelled 5′-m(7)G-capped mRNA from two genes, the simple mono-exonic Beta-2-Adrenoceptor (AD...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4824077/ https://www.ncbi.nlm.nih.gov/pubmed/26615195 http://dx.doi.org/10.1093/nar/gkv1328 |