Where does transcription start? 5′-RACE adapted to next-generation sequencing

The variability and complexity of the transcription initiation process was examined by adapting RNA ligase-mediated rapid amplification of 5′ cDNA ends (5′-RACE) to Next-Generation Sequencing (NGS). We oligo-labelled 5′-m(7)G-capped mRNA from two genes, the simple mono-exonic Beta-2-Adrenoceptor (AD...

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Detalles Bibliográficos
Autores principales: Leenen, Fleur A.D., Vernocchi, Sara, Hunewald, Oliver E., Schmitz, Stephanie, Molitor, Anne M., Muller, Claude P., Turner, Jonathan D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2016
Materias:
Gene regulation, Chromatin and Epigenetics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4824077/
https://www.ncbi.nlm.nih.gov/pubmed/26615195
http://dx.doi.org/10.1093/nar/gkv1328

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