Chemo-enzymatic synthesis of site-specific isotopically labeled nucleotides for use in NMR resonance assignment, dynamics and structural characterizations

Stable isotope labeling is central to NMR studies of nucleic acids. Development of methods that incorporate labels at specific atomic positions within each nucleotide promises to expand the size range of RNAs that can be studied by NMR. Using recombinantly expressed enzymes and chemically synthesize...

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Detalles Bibliográficos
Autores principales: Longhini, Andrew P., LeBlanc, Regan M., Becette, Owen, Salguero, Carolina, Wunderlich, Christoph H., Johnson, Bruce A., D'Souza, Victoria M., Kreutz, Christoph, Dayie, T. Kwaku
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2016
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4824079/
https://www.ncbi.nlm.nih.gov/pubmed/26657632
http://dx.doi.org/10.1093/nar/gkv1333

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4824079/
https://www.ncbi.nlm.nih.gov/pubmed/26657632
http://dx.doi.org/10.1093/nar/gkv1333

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