Improved methods of AAV-mediated gene targeting for human cell lines using ribosome-skipping 2A peptide

The adeno-associated virus (AAV)-based targeting vector has been one of the tools commonly used for genome modification in human cell lines. It allows for relatively efficient gene targeting associated with 1–4-log higher ratios of homologous-to-random integration of targeting vectors (H/R ratios) t...

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Detalles Bibliográficos
Autores principales: Karnan, Sivasundaram, Ota, Akinobu, Konishi, Yuko, Wahiduzzaman, Md, Hosokawa, Yoshitaka, Konishi, Hiroyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2016
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4824082/
https://www.ncbi.nlm.nih.gov/pubmed/26657635
http://dx.doi.org/10.1093/nar/gkv1338

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4824082/
https://www.ncbi.nlm.nih.gov/pubmed/26657635
http://dx.doi.org/10.1093/nar/gkv1338

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