iSpinach: a fluorogenic RNA aptamer optimized for in vitro applications

Using random mutagenesis and high throughput screening by microfluidic-assisted In Vitro Compartmentalization, we report the isolation of an order of magnitude times brighter mutants of the light-up RNA aptamers Spinach that are far less salt-sensitive and with a much higher thermal stability than t...

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Detalles Bibliográficos
Autores principales: Autour, Alexis, Westhof, Eric, Ryckelynck, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2016
Materias:
Chemical Biology and Nucleic Acid Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4824111/
https://www.ncbi.nlm.nih.gov/pubmed/26932363
http://dx.doi.org/10.1093/nar/gkw083

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4824111/
https://www.ncbi.nlm.nih.gov/pubmed/26932363
http://dx.doi.org/10.1093/nar/gkw083

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