Kinetics of the CRISPR-Cas9 effector complex assembly and the role of 3′-terminal segment of guide RNA

CRISPR-Cas9 is widely applied for genome engineering in various organisms. The assembly of single guide RNA (sgRNA) with the Cas9 protein may limit the Cas9/sgRNA effector complex function. We developed a FRET-based assay for detection of CRISPR–Cas9 complex binding to its targets and used this assa...

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Detalles Bibliográficos
Autores principales: Mekler, Vladimir, Minakhin, Leonid, Semenova, Ekaterina, Kuznedelov, Konstantin, Severinov, Konstantin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2016
Materias:
Nucleic Acid Enzymes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4824121/
https://www.ncbi.nlm.nih.gov/pubmed/26945042
http://dx.doi.org/10.1093/nar/gkw138

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4824121/
https://www.ncbi.nlm.nih.gov/pubmed/26945042
http://dx.doi.org/10.1093/nar/gkw138

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