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A Plasmid Set for Efficient Bacterial Artificial Chromosome (BAC) Transgenesis in Zebrafish

Transgenesis of large DNA constructs is essential for gene function analysis. Recently, Tol2 transposase-mediated transgenesis has emerged as a powerful tool to insert bacterial artificial chromosome (BAC) DNA constructs into the genome of zebrafish. For efficient transgenesis, the genomic DNA piece...

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Detalles Bibliográficos
Autores principales: Fuentes, Fernando, Reynolds, Eric, Lewellis, Stephen W., Venkiteswaran, Gayatri, Knaut, Holger
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4825653/
https://www.ncbi.nlm.nih.gov/pubmed/26818072
http://dx.doi.org/10.1534/g3.115.026344