A Recombinant Secondary Antibody Mimic as a Target-specific Signal Amplifier and an Antibody Immobilizer in Immunoassays

We construct a novel recombinant secondary antibody mimic, GST-ABD, which can bind to the Fc regions of target-bound primary antibodies and acquire multiple HRPs simultaneously. We produce it in tenth of mg quantities with a bacterial overexpression system and simple purification procedures, signifi...

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Detalles Bibliográficos
Autores principales: Min, Junseon, Song, Eun Kyung, Kim, Hansol, Kim, Kyoung Taek, Park, Tae Joo, Kang, Sebyung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4827060/
https://www.ncbi.nlm.nih.gov/pubmed/27063487
http://dx.doi.org/10.1038/srep24159

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4827060/
https://www.ncbi.nlm.nih.gov/pubmed/27063487
http://dx.doi.org/10.1038/srep24159

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