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A Recombinant Secondary Antibody Mimic as a Target-specific Signal Amplifier and an Antibody Immobilizer in Immunoassays
We construct a novel recombinant secondary antibody mimic, GST-ABD, which can bind to the Fc regions of target-bound primary antibodies and acquire multiple HRPs simultaneously. We produce it in tenth of mg quantities with a bacterial overexpression system and simple purification procedures, signifi...
Autores principales: | Min, Junseon, Song, Eun Kyung, Kim, Hansol, Kim, Kyoung Taek, Park, Tae Joo, Kang, Sebyung |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4827060/ https://www.ncbi.nlm.nih.gov/pubmed/27063487 http://dx.doi.org/10.1038/srep24159 |
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