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Evaluation of microbial qPCR workflows using engineered Saccharomyces cerevisiae

AIMS: We describe the development and interlaboratory study of modified Saccharomyces cerevisiae as a candidate material to evaluate a full detection workflow including DNA extraction and quantitative polymerase chain reaction (qPCR). METHODS AND RESULTS: S. cerevisiae NE095 was prepared by stable i...

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Detalles Bibliográficos
Autores principales: Da Silva, S.M., Vang, L.K., Olson, N.D., Lund, S.P., Downey, A.S., Kelman, Z., Salit, M.L., Lin, N.J., Morrow, J.B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4827694/
https://www.ncbi.nlm.nih.gov/pubmed/27077050
http://dx.doi.org/10.1016/j.bdq.2016.01.001