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Evaluation of microbial qPCR workflows using engineered Saccharomyces cerevisiae
AIMS: We describe the development and interlaboratory study of modified Saccharomyces cerevisiae as a candidate material to evaluate a full detection workflow including DNA extraction and quantitative polymerase chain reaction (qPCR). METHODS AND RESULTS: S. cerevisiae NE095 was prepared by stable i...
Autores principales: | Da Silva, S.M., Vang, L.K., Olson, N.D., Lund, S.P., Downey, A.S., Kelman, Z., Salit, M.L., Lin, N.J., Morrow, J.B. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4827694/ https://www.ncbi.nlm.nih.gov/pubmed/27077050 http://dx.doi.org/10.1016/j.bdq.2016.01.001 |
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